Linked Life Data

17387746

Source:http://linkedlifedata.com/resource/pubmed/id/17387746

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2007-4-19
pubmed:abstractText
Presynaptic boutons and associated postsynaptic structures in the CNS express markers that are highly synapse type-specific. In multilabel immunofluorescence imaging, coexpression of such markers appears as overlap of signals in the same structures whereas closely related yet segregated markers, e.g., located pre-and postsynaptically, translate into signals that touch. 'Overlap' and 'touch' occur in three dimensions (3D). The instrument of choice to study overlap vs. touch of small objects in tissue volumes is the confocal laser scanning microscope (CSLM). To quantify overlap and touch we used two paradigms. Overlap was studied in rat brain sections triple-immunostained with antibodies against markers predominantly located presynaptically: glutamic acid decarboxylase, vesicular glutamate transporter 2, and calretinin. Touch was studied in rat temporal cortex where afferent, tracer-labeled entorhinohippocampal fibers in the subiculum were imaged together with possible postsynaptic target neurons immunostained with an antibody against the calcium binding protein, parvalbumin. Z-series of CLSM images were obtained in multiple channels. After post-acquisition deconvolution we further processed the images via software written in the C/C++ SCIL Image computer programming environment. The software receives parameters via scripts, after which it first identifies 3D objects through establishing isodensity envelopes around pixels representing small biologic structures (in our case: boutons) and then compares associated Z-series in which it determines whether there is overlap or touch between recognized 3D objects. Finally, graphic and numeric output is produced. With this script-commanded software we feel equipped to accurately and objectively quantify overlap and touch.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0360-4012
pubmed:author
pubmed:copyrightInfo
(c) 2007 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1215-28
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Computer-assisted estimation in the CNS of 3D multimarker 'overlap' or 'touch' at the level of individual nerve endings: a confocal laser scanning microscope application.
pubmed:affiliation
Department of Anatomy and Neurosciences, VU University Medical Center, Amsterdam, The Netherlands. fg.wouterlood@vumc.nl
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't