Linked Life Data

17386514

Source:http://linkedlifedata.com/resource/pubmed/id/17386514

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-3-27
pubmed:abstractText
A novel method for the immobilization of hemoglobin (Hb) and preparation of reagentless biosensor was proposed using a biocompatible non-toxic zirconia enhanced grafted collagen tri-helix scaffold. The formed membrane was characterized with UV-vis and FT-IR spectroscopy, scanning electron microscope and electrochemical methods. The Hb immobilized in the matrix showed excellent direct electrochemistry with an electron transfer rate constant of 6.46 s(-1) and electrocatalytic activity to the reduction of hydrogen peroxide. The apparent Michaelis-Menten constant for H(2)O(2) was 0.026 mM, showing good affinity. Based on the direct electrochemistry, a new biosensor for H(2)O(2) ranging from 0.8 to 132 microM was constructed. Owing to the porous structure and high enzyme loading of the matrix the biosensor exhibited low limit of detection of 0.12 microM at 3sigma, fast response less than 5 s and high sensitivity of 45.6 mA M(-1) cm(-2). The biosensor exhibited acceptable stability and reproducibility. ZrO(2)-grafted collagen provided a good matrix for protein immobilization and biosensing preparation. This method was useful for monitoring H(2)O(2) in practical samples with the satisfactory results.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1873-4324
pubmed:author
pubmed:issnType
Electronic
pubmed:day
23
pubmed:volume
582
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
361-6
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Hydrogen peroxide biosensor based on hemoglobin modified zirconia nanoparticles-grafted collagen matrix.
pubmed:affiliation
MOE Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China; Department of Chemistry, Changshu Institute of Technology, Changshu 215500, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't