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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-3-18
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pubmed:abstractText |
Iron regulatory factor (IRF), also called iron responsive element-binding protein (IRE-BP), is a cytoplasmic RNA-binding protein which regulates post-transcriptionally transferrin receptor mRNA stability and ferritin mRNA translation. By using the polymerase chain reaction (PCR) and the sequence published by Rouault et al. (1990) a probe was derived which permitted the isolation of three human IRF cDNA clones. Hybridization to genomic DNA and mRNA, as well as sequencing data indicated a single copy gene of about 40 kb specifying a 4.0 kb mRNA that translates into a protein of 98,400 dalton. By in vitro transcription of a assembled IRF cDNA coupled to in vitro translation in a wheat germ extract, we obtained full sized IRF that bound specifically to a human ferritin IRE. In vitro translated IRF retained sensitivity to sulfhydryl oxidation by diamide and could be reactivated by beta-mercaptoethanol in the same way as native placental IRF. An IRF deletion mutant shortened by 132 amino acids at the COOH-terminus was no longer able to bind to an IRE, indicating that this region of the protein plays a role in RNA recognition. Placental IRF has previously been shown to migrate as a doublet on SDS-polyacrylamide gels. After V8 protease digestion the heterogeneity was located in a 65/70 kDa NH2-terminal doublet. The liberated 31 kDa COOH-terminal polypeptide was found to be homogeneous by amino acid sequencing supporting the conclusion of a single IRF gene.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-1083028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-1701143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-1903202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2001588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2050125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2050126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2095458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2106665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2157191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2169889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2172968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2294594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2324109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2376574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2418417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2421882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2452485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2470643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2474819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2498873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2569464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2583116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2598939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2601708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2711187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2752428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2760024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2771641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-2828872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-3036867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-3050531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-3127826,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-3370673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-3413484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-3477805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-3479802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-3608980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-6309829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-6343825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1738601-6680152
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
20
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pubmed:geneSymbol |
IRF
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
33-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1738601-Amino Acid Sequence,
pubmed-meshheading:1738601-Base Sequence,
pubmed-meshheading:1738601-Blotting, Northern,
pubmed-meshheading:1738601-Blotting, Southern,
pubmed-meshheading:1738601-Cloning, Molecular,
pubmed-meshheading:1738601-Endopeptidases,
pubmed-meshheading:1738601-Ferritins,
pubmed-meshheading:1738601-Gene Expression,
pubmed-meshheading:1738601-Humans,
pubmed-meshheading:1738601-Iron-Regulatory Proteins,
pubmed-meshheading:1738601-Molecular Sequence Data,
pubmed-meshheading:1738601-RNA, Messenger,
pubmed-meshheading:1738601-RNA-Binding Proteins,
pubmed-meshheading:1738601-Receptors, Transferrin,
pubmed-meshheading:1738601-Trypsin
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pubmed:year |
1992
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pubmed:articleTitle |
Expression of active iron regulatory factor from a full-length human cDNA by in vitro transcription/translation.
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