Linked Life Data

17379376

Source:http://linkedlifedata.com/resource/pubmed/id/17379376

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2007-4-9
pubmed:abstractText
The aim of this study was to identify the di(n-butyl) phthalate (DBP)-induced differentially expressed genes (DEGs) using a novel annealing control primer system in the testes of Sprague-Dawley male rats. Animals (4 weeks of age) were administered orally either corn oil only (vehicle control) or DBP (250, 500, or 750 mg/kg/day) for 30 days. Total RNA was isolated from the rat testes and GeneFishing PCR was used to determine the differential gene expression levels. Using this technique, a total of 59 DEG mRNA fragments were observed in the testes treated with DBP 750 mg/kg/day compared to vehicle control. Of these 59 genes, 31 genes were significantly altered after exposing rats to high dose DBP (750 mg/kg/day), and their sequences cloned. Based on the Basic Local Alignment Search Tool (BLAST), 4 expressed sequence tags (EST), 27 cloned genes (Insl3, pgrp, H1SHR, etc.) and 3 genes (LDHA, lactate dehydrogenase A; Spag4, sperm associated antigen 4 and PBR, peripheral-type benzodiazepine receptor) were found to be involved in spermatogenesis and steroidogenesis. In addition, the expression patterns of the steroidogenesis-related genes such as scavenger receptor class B-1 (SR-B1), steroidogenic acute regulated protein (StAR), P450 side chain cleavage (P450scc), CYP17, and CYP19 were further analyzed by RT-PCR. Significant increases in the mRNA levels of steroidogenesis-related genes (PBR, SR-B1, StAR, P450scc, and CYP17) were observed in the high dose DBP-treated rats. However, DBP significantly decreased the CYP19 mRNA levels compared with controls. DBP (750 mg/kg/day) significantly increased the TR-alpha1 and PPARgamma expression in testes, whereas the AR and ERbeta protein levels were significantly reduced in the same group. These data indicate that the steroidogenesis- or spermatogenesis-related genes identified in this study may provide insights into the molecular mechanisms underlying environmental pollutants-mediated male infertility.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0300-483X
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
234
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
103-12
pubmed:meshHeading
pubmed-meshheading:17379376-Administration, Oral, pubmed-meshheading:17379376-Age Factors, pubmed-meshheading:17379376-Animals, pubmed-meshheading:17379376-Blotting, Western, pubmed-meshheading:17379376-Dibutyl Phthalate, pubmed-meshheading:17379376-Dose-Response Relationship, Drug, pubmed-meshheading:17379376-Estrogen Receptor beta, pubmed-meshheading:17379376-Gene Expression Profiling, pubmed-meshheading:17379376-Gene Expression Regulation, pubmed-meshheading:17379376-Genes, erbA, pubmed-meshheading:17379376-Male, pubmed-meshheading:17379376-Organ Size, pubmed-meshheading:17379376-PPAR alpha, pubmed-meshheading:17379376-Plasticizers, pubmed-meshheading:17379376-RNA, Messenger, pubmed-meshheading:17379376-Rats, pubmed-meshheading:17379376-Rats, Sprague-Dawley, pubmed-meshheading:17379376-Receptors, Androgen, pubmed-meshheading:17379376-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:17379376-Spermatogenesis, pubmed-meshheading:17379376-Testis
pubmed:year
2007
pubmed:articleTitle
Identification of differentially expressed genes in the testis of Sprague-Dawley rats treated with di(n-butyl) phthalate.
pubmed:affiliation
Laboratory of Molecular Toxicology, College of Pharmacy, Pusan National University, San 30, Jangjun-Dong, Gumjung-Ku, Busan, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't