Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2007-5-14
pubmed:abstractText
Epstein-Barr virus (EBV) causes hairy leukoplakia (HL), a benign lesion of oral epithelium that occurs primarily in the setting of human immunodeficiency virus (HIV)-associated immunodeficiency. However, the mechanisms of EBV infection of oral epithelium are poorly understood. Analysis of HL tissues shows a small number of EBV-positive intraepithelial macrophages and dendritic/Langerhans cells. To investigate a role for these cells in spreading EBV to epithelial cells, we used tongue and buccal explants infected ex vivo with EBV. We showed that EBV first infects submucosal CD14(+) monocytes, which then migrate into the epithelium and spread virus to oral epithelial cells, initiating productive viral infection within the terminally differentiated spinosum and granulosum layers. Incubation of EBV-infected monocytes and oral explants with antibodies to CCR2 receptor and monocyte chemotactic protein 1 prevented entry of monocytes into the epithelium and inhibited EBV infection of keratinocytes. B lymphocytes played little part in the spread of EBV to keratinocytes in our explant model. However, cocultivation of EBV-infected B lymphocytes with uninfected monocytes in vitro showed that EBV may spread from B lymphocytes to monocytes. Circulating EBV-positive monocytes were detected in most HIV-infected individuals, consistent with a model in which EBV may be spread from B lymphocytes to monocytes, which then enter the epithelium and initiate productive viral infection of keratinocytes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-10076516, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-10684275, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-10775673, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-10837075, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-10906215, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-11364217, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-11389046, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-11462026, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-11807224, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-11861876, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-11889060, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-12050358, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-12164661, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-12485331, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-12592401, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-1316871, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-15024179, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-15078925, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-15173832, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-15448337, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-15507678, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-15613356, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-15654989, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-15872054, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-16385921, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-1646280, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-16476057, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-16606841, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-1662695, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-1851858, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-2847171, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-2999595, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-3110300, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-3492574, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-7714494, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-7817670, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-8393230, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-8853725, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-9287323, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-9311888, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-9563407, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-9774522, http://linkedlifedata.com/resource/pubmed/commentcorrection/17376918-9848893
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5484-96
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Epstein-Barr virus (EBV)-infected monocytes facilitate dissemination of EBV within the oral mucosal epithelium.
pubmed:affiliation
Department of Medicine, University of California San Francisco, 513 Parnassus Ave., San Francisco, CA 94143-0512, USA. sharof.tugizov@ucsf.edu
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural