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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-3-17
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pubmed:abstractText |
The haptoglobin-related gene product (HGRP) having greater than 90% sequence homology with plasma haptoglobin, has recently been implicated as a tumor maker of recurrent breast cancer. Therefore, availability of a monoclonal antibody which is specific to the HGRP and ensuing ELISAs to quantitate the HGRP without cross-reactivity with plasma haptoglobin will allow determination of the clinical value of the HGRP in human plasma under various pathophysiological conditions. A peptide representing the first 34 residues of the N-terminal portion of the HRGP was synthesized and conjugated to keyhole limpet hemocyanin (KLH) to immunize Balb/c mice. Hybrid clones that produced specific antibodies to HRGP were screened with the same peptide but conjugated to bovine serum albumin (HRGP-BSA). From 12 hybridoma clones that recognized HGRP-peptide, we obtained one IgM producing clone (27.5) by limiting dilution technique that selectively reacted with HGRP-peptide with minimal cross-reactivity with haptoglobin. Using a separate monoclonal antibody, clone 21.7, which is directed to the alpha subunit of haptoglobin, we developed an enzyme-linked immunosorbent assay (ELISA) to determine the quantities of the HGRP in human plasma, which will allow assessment of HGRP as a clinical marker of malignancy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HPR protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Haptoglobins,
http://linkedlifedata.com/resource/pubmed/chemical/Hemocyanin,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin, Bovine,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/keyhole-limpet hemocyanin
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0272-457X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-12
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1737636-Adolescent,
pubmed-meshheading:1737636-Adult,
pubmed-meshheading:1737636-Aged,
pubmed-meshheading:1737636-Aged, 80 and over,
pubmed-meshheading:1737636-Animals,
pubmed-meshheading:1737636-Antibodies, Monoclonal,
pubmed-meshheading:1737636-Antigens, Neoplasm,
pubmed-meshheading:1737636-Blood Proteins,
pubmed-meshheading:1737636-Blotting, Western,
pubmed-meshheading:1737636-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1737636-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1737636-Female,
pubmed-meshheading:1737636-Goats,
pubmed-meshheading:1737636-Haptoglobins,
pubmed-meshheading:1737636-Hemocyanin,
pubmed-meshheading:1737636-Humans,
pubmed-meshheading:1737636-Hybridomas,
pubmed-meshheading:1737636-Male,
pubmed-meshheading:1737636-Mice,
pubmed-meshheading:1737636-Mice, Inbred BALB C,
pubmed-meshheading:1737636-Middle Aged,
pubmed-meshheading:1737636-Mollusca,
pubmed-meshheading:1737636-Rabbits,
pubmed-meshheading:1737636-Serum Albumin, Bovine,
pubmed-meshheading:1737636-Tumor Markers, Biological
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pubmed:year |
1992
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pubmed:articleTitle |
Quantitative differentiation of the haptoglobin-related gene product from haptoglobin in human plasma: a possible test for tumor-associated antigen.
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pubmed:affiliation |
Department of Microbiology, Boston University School of Medicine, MA 02118.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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