Linked Life Data

17376164

Source:http://linkedlifedata.com/resource/pubmed/id/17376164

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-4-20
pubmed:abstractText
Wax biosynthetic pathways proceed via the elongation of 16:0 acyl-CoA to very long-chain fatty acids (VLCFA), and by further modifications that include reduction to primary alcohols and formation of alkyl esters. We have analyzed the alkyl esters in the stem wax of ten cer mutants of Arabidopsis thaliana together with the corresponding wild types. Alkyl esters with chain lengths between C(38) and C(52) were identified, and the levels of esters ranged from 0.15 microg cm(-2) in Wassilewskija (WS) to 1.20 microg cm(-2) in cer2. Esters with even numbers of carbons prevailed, with C(42), C(44) and C(46) favoured in the wild types, a predominance of C(42) in cer2 and cer6 mutants, and a relative shift towards C(46) in cer3 and cer23 mutants. The esters of all mutants and wild types were dominated by 16:0 acyl moieties, whereas the chain lengths of esterified alcohols were between C(20) and C(32). The alkyl chain-length distributions of the wild-type esters had a maximum for C(28) alcohol, similar to the free alcohols accompanying them in the wax mixtures. The esterified alcohols of cer2, cer6 and cer9 had largely increased levels of C(26) alcohol, closely matching the patterns of the corresponding free alcohols and, therefore, differing drastically from the corresponding wild type. In contrast, cer1, cer3, cer10, cer13 and cer22 showed ester alcohol patterns with increased levels of C(30), only partially following the shift in chain lengths of the free alcohols in stem wax. These results provide information on the composition of substrate pools and/or the specificity of the ester synthase involved in wax ester formation. We conclude that alcohol levels at the site of biosynthesis are mainly limiting the ester formation in the Arabidopsis wild-type epidermis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0960-7412
pubmed:author
pubmed:issnType
Print
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
189-96
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Composition of alkyl esters in the cuticular wax on inflorescence stems of Arabidopsis thaliana cer mutants.
pubmed:affiliation
Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, BC, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't