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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2007-5-16
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pubmed:abstractText |
This work constitutes the first fluorescent imaging of cells using metal plasmon-coupled probes (PCPs) at single cell resolution. N-(2-Mercapto-propionyl)glycine-coated silver nanoparticles were synthesized by reduction of silver nitrate using sodium borohyride and then succinimidylated via ligand exchange. Alexa Fluor 647-labeled concanavalin A (con A) was chemically bound to the silver particles to make the fluorescent metal plasmon-coupled probes. The fluorescence images were collected using a scanning confocal microscopy. The fluorescence intensity was observed to enhance 7-fold when binding the labeled con A on a single silver particle. PCPs were conjugated on HEK 293 A cells. Imaging results demonstrate that cells labeled by PCPs were 20-fold brighter than those by free labeled con A.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/EB-00682,
http://linkedlifedata.com/resource/pubmed/grant/HG-02655,
http://linkedlifedata.com/resource/pubmed/grant/P41 RR008119-13,
http://linkedlifedata.com/resource/pubmed/grant/P41 RR008119-14,
http://linkedlifedata.com/resource/pubmed/grant/R01 EB000682-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 EB000682-04,
http://linkedlifedata.com/resource/pubmed/grant/R01 EB000682-05,
http://linkedlifedata.com/resource/pubmed/grant/R01 HG002655-03,
http://linkedlifedata.com/resource/pubmed/grant/R01 HG002655-04A1,
http://linkedlifedata.com/resource/pubmed/grant/R01 HG002655-05,
http://linkedlifedata.com/resource/pubmed/grant/RR-08119
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-10207887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-10639073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-11250744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-11673890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-11971705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-12141663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-12405861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-12672412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-15080730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-15144206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-15264814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-15301553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-15453801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-15691498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-15722013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-15826019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-15826096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-15897449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-16028894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-16089488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-16144364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-16159249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-16351199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-16390096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-16568977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-16851429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-16851886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-16851931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17375898-16853202
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1043-1802
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
800-5
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pubmed:dateRevised |
2011-5-17
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pubmed:meshHeading |
pubmed-meshheading:17375898-Cells, Cultured,
pubmed-meshheading:17375898-Concanavalin A,
pubmed-meshheading:17375898-Cyclic AMP,
pubmed-meshheading:17375898-Fluorescence,
pubmed-meshheading:17375898-Humans,
pubmed-meshheading:17375898-Metal Nanoparticles,
pubmed-meshheading:17375898-Microscopy, Confocal,
pubmed-meshheading:17375898-Microscopy, Fluorescence,
pubmed-meshheading:17375898-Molecular Probes,
pubmed-meshheading:17375898-Silver,
pubmed-meshheading:17375898-Surface Plasmon Resonance,
pubmed-meshheading:17375898-Tiopronin
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pubmed:articleTitle |
Single cell fluorescence imaging using metal plasmon-coupled probe.
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pubmed:affiliation |
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry and Molecular Biology, 725 West Lombard Street, Baltimore, Maryland 21201, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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