17373475

Source:http://linkedlifedata.com/resource/pubmed/id/17373475

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0010819, umls-concept:C0185125, umls-concept:C1510438
pubmed:issue	2
pubmed:dateCreated	2007-3-21
pubmed:abstractText	We have developed a general experimental strategy that enables the quantitative detection of dynamic protein-protein interactions in intact living cells, based on protein-fragment complementation assays (PCAs). In this method, protein-protein interactions are coupled to refolding of enzymes from cognate fragments where reconstitution of enzyme activity acts as the detector of a protein interaction. Here we discuss the application of PCA to different aspects of cell biology.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/8306785
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Proteins
pubmed:status	MEDLINE
pubmed:month	Feb
pubmed:issn	0736-6205
pubmed:author	pubmed-author:MichnickStephen WSW, pubmed-author:RemyIngridI
pubmed:issnType	Print
pubmed:volume	42
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	137, 139, 141 passim
pubmed:dateRevised	2009-11-19
pubmed:meshHeading	pubmed-meshheading:17373475-Biochemical Phenomena, pubmed-meshheading:17373475-Biochemistry, pubmed-meshheading:17373475-Cell Biology, pubmed-meshheading:17373475-Proteins
pubmed:year	2007
pubmed:articleTitle	Application of protein-fragment complementation assays in cell biology.
pubmed:affiliation	Département de Biochimie, Université de Montréal, Succursale Center-Ville, Montréal, QC, Canada.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't