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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2007-3-20
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pubmed:abstractText |
In humans, the pathways of memory and effector T cell differentiation remain poorly defined. We have dissected the functional properties of ex vivo effector-memory (EM) CD45RA-CCR7- T lymphocytes present within the circulating CD8+ T cell pool of healthy individuals. Our studies show that EM T cells are heterogeneous and are subdivided based on differential CD27 and CD28 expression into four subsets. EM(1) (CD27+CD28+) and EM(4) (CD27-CD28+) T cells express low levels of effector mediators such as granzyme B and perforin and high levels of CD127/IL-7Ralpha. EM(1) cells also have a relatively short replicative history and display strong ex vivo telomerase activity. Therefore, these cells are closely related to central-memory (CD45RA-CCR7+) cells. In contrast, EM(2) (CD27+CD28-) and EM(3) (CD27-CD28-) cells express mediators characteristic of effector cells, whereby EM(3) cells display stronger ex vivo cytolytic activity and have experienced larger numbers of cell divisions, thus resembling differentiated effector (CD45RA+CCR7-) cells. These data indicate that progressive up-regulation of cytolytic activity and stepwise loss of CCR7, CD28, and CD27 both characterize CD8+ T cell differentiation. Finally, memory CD8+ T cells not only include central-memory cells but also EM(1) cells, which differ in CCR7 expression and may therefore confer memory functions in lymphoid and peripheral tissues, respectively.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD27,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/CCR6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Granzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-7 Receptor alpha Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Perforin,
http://linkedlifedata.com/resource/pubmed/chemical/Pore Forming Cytotoxic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, CCR6,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Chemokine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-7,
http://linkedlifedata.com/resource/pubmed/chemical/interleukin-7 receptor, alpha chain
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
178
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4112-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:17371966-Adult,
pubmed-meshheading:17371966-Aged,
pubmed-meshheading:17371966-Antigens, CD27,
pubmed-meshheading:17371966-Antigens, CD28,
pubmed-meshheading:17371966-CD8-Positive T-Lymphocytes,
pubmed-meshheading:17371966-Cell Differentiation,
pubmed-meshheading:17371966-Female,
pubmed-meshheading:17371966-Flow Cytometry,
pubmed-meshheading:17371966-Gene Expression Profiling,
pubmed-meshheading:17371966-Granzymes,
pubmed-meshheading:17371966-Humans,
pubmed-meshheading:17371966-Immunologic Memory,
pubmed-meshheading:17371966-Interleukin-7 Receptor alpha Subunit,
pubmed-meshheading:17371966-Male,
pubmed-meshheading:17371966-Membrane Glycoproteins,
pubmed-meshheading:17371966-Middle Aged,
pubmed-meshheading:17371966-Perforin,
pubmed-meshheading:17371966-Pore Forming Cytotoxic Proteins,
pubmed-meshheading:17371966-RNA, Messenger,
pubmed-meshheading:17371966-Receptors, Antigen, T-Cell,
pubmed-meshheading:17371966-Receptors, CCR6,
pubmed-meshheading:17371966-Receptors, Chemokine,
pubmed-meshheading:17371966-Receptors, Interleukin-7,
pubmed-meshheading:17371966-T-Lymphocyte Subsets,
pubmed-meshheading:17371966-Telomere
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pubmed:year |
2007
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pubmed:articleTitle |
Four functionally distinct populations of human effector-memory CD8+ T lymphocytes.
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pubmed:affiliation |
Division of Clinical Onco-Immunology, Ludwig Institute for Cancer Research-Lausanne Branch, University Hospital of Lausanne, Lausanne, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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