Linked Life Data

17368796

Source:http://linkedlifedata.com/resource/pubmed/id/17368796

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-6-11
pubmed:abstractText
The utility of real-time polymerase chain reaction (RT-PCR) testing for detection of methicillin-resistant Staphylococcus aureus (MRSA) directly from positive blood culture bottles was evaluated. One hundred forty-two blood cultures showing Gram-positive cocci in clusters were detected. Each blood culture sample was tested for the presence of MRSA by PCR analysis (SmartCycler) via detection of the mecA and orfX genes. In parallel, they were plated on standard media for identification and characterization. PCR analysis directly from the blood culture bottle required a total time of 120 min (45 min for preparation and 75 min for the reaction). By comparison, conventional laboratory procedures required between 48 and 72 h. The overall test accuracy was 97% with a high positive likelihood ratio and a low negative likelihood ratio.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0732-8893
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
199-202
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Use of real-time polymerase chain reaction for identification of methicillin-resistant Staphylococcus aureus directly from positive blood culture bottles.
pubmed:affiliation
Department of Laboratory Medicine, Yale University School of Medicine, P.O. Box 208035, New Haven, CT 06520-8035, USA.
pubmed:publicationType
Journal Article, Evaluation Studies