Linked Life Data

17367967

Source:http://linkedlifedata.com/resource/pubmed/id/17367967

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2007-5-28
pubmed:abstractText
Polyethyleneimine nanogels (named as M-PEIs) with different sizes were prepared by photo-Fenton reaction in aqueous solution from which samples of 38, 75, 87, 121, 132 and 167nm were selected for in vitro transfection. The homogeneous structure and the same component made it possible to study the size effect of M-PEIs nanogels on gene transfection efficiency when loading the same quantity of plasmid DNA (pLEGFP-C1) into A549, Bel7402, BGC-823 and Hela cells. M-PEIs and its DNA complexes were characterized by photo correlation spectroscopy and atomic force microscopy. The protein expression was observed by flow cytometry and fluorescence microscopy. All of the DNA complexes had no obvious cytotoxicity and the surface charges were positive charged at the optimum weight ratio. Therefore, the expressed protein was affected by the size of M-PEIs when the same quantity of DNA was used to transfect cells. In addition, the samples of 75 and 87nm yielded the highest transfection efficiency about 30% in all of the four cell lines which were also cell line independent.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0378-5173
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
338
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
291-6
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Size-dependent properties of M-PEIs nanogels for gene delivery in cancer cells.
pubmed:affiliation
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't