Source:http://linkedlifedata.com/resource/pubmed/id/17365856
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2007-3-16
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| pubmed:abstractText |
Platelet activation in preeclampsia is reflected by elevated levels of platelets exposing P-selectin. In plasma, a non-cell bound (soluble) form of P-selectin is present. Elevated levels of this soluble form have been reported in preeclampsia. Plasma P-selectin may consist of two fractions: microparticle (MP)--associated P-selectin and non-MP--associated P-selectin. In the present cross-sectional study, we investigated to which extent plasma P-selectin is MP--associated and whether such MP are elevated in preeclamptic patients. Preeclamptic patients (n=10) were matched with normotensive pregnant women (n=10) and non-pregnant controls (n=10). Plasma P-selectin was measured by ELISA. MP were isolated, double labelled with anti-CD61 (GPIIIa) and anti-CD62P (P-selectin) and subsequently analyzed with flowcytometry. Plasma P-selectin concentration was elevated in preeclamptic patients compared to non-pregnant controls (p=0.007), but not compared to normotensive pregnant women (p=0.210). Plasma P-selectin is partially MP--associated (3-5%). In pregnancy, the fraction of P-selectin exposing platelet-derived MP (PMP) (10.9%) was increased compared to non-pregnant controls (8%). This fraction further increased in preeclamptic patients (15.4%), and significantly differed from normotensive pregnant women (p=0.02). A minor fraction of plasma P-selectin is associated with PMP. The fraction of PMP exposing P-selectin is increased in preeclamptic patients and to a lesser extent in normotensive pregnancy. Because MP associated P-selectin exclusively originates from platelets, this fraction indicates platelet activation. Platelet activation is prominent in preeclampsia and this study proves that at least a part of the plasma P-selectin originates from platelets.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0953-7104
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
18
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
68-72
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| pubmed:meshHeading |
pubmed-meshheading:17365856-Adult,
pubmed-meshheading:17365856-Anemia, Hemolytic,
pubmed-meshheading:17365856-Blood Platelets,
pubmed-meshheading:17365856-Case-Control Studies,
pubmed-meshheading:17365856-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:17365856-Female,
pubmed-meshheading:17365856-Humans,
pubmed-meshheading:17365856-P-Selectin,
pubmed-meshheading:17365856-Particle Size,
pubmed-meshheading:17365856-Platelet Activation,
pubmed-meshheading:17365856-Pre-Eclampsia,
pubmed-meshheading:17365856-Pregnancy,
pubmed-meshheading:17365856-Pregnancy Complications, Hematologic,
pubmed-meshheading:17365856-Syndrome,
pubmed-meshheading:17365856-Thrombocytopenia,
pubmed-meshheading:17365856-Thrombophilia
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| pubmed:year |
2007
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| pubmed:articleTitle |
Microparticle-associated P-selectin reflects platelet activation in preeclampsia.
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| pubmed:affiliation |
Department of Obstetrics and Gynecology, Academic Medical Centre, Amsterdam, The Netherlands. c.a.lok@amc.uva.nl
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| pubmed:publicationType |
Journal Article
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