Linked Life Data

17363202

Source:http://linkedlifedata.com/resource/pubmed/id/17363202

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2007-5-28
pubmed:abstractText
Glycol chitosan (GC), a chitosan derivative conjugated with ethylene glycol, is soluble in water at a neutral/acidic pH and is viscous. This GC was incorporated into poly(lactide-co-glycolide) (PLGA) microparticles (prepared by the multi-emulsion W(1)/O/W(2) (water-in-oil-in-water) method) to stabilize lysozyme (Lys) used as a model protein. Herein, GC's viscous property helped to improve Lys encapsulation efficacy and reduce Lys denaturaton at the water/organic solvent interface. When the GC concentration in the W(1) phase increased, the formation of non-covalent Lys aggregates decreased. This may be because the aqueous microdroplets surrounded by the firm viscous interface protect Lys from the degrading environment formed by the water/organic solvent interface. In an in vitro Lys release test, 40mg incorporation of GC led to continuous Lys release of up to 78wt.% for 1 month and presented bioactivity of more than 95% for Lys released from microparticles. In addition, there was negligible immune response in the tissue treated with the GC-incorporated PLGA microparticles, whereas there was a moderate foreign body reaction in the muscle layer and many configurations of neutrophils in the tissue treated with the PLGA microparticles without GC. It is expected that GC facilitates a decrease in immune responses exacerbated as a consequence of PLGA degradation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0378-5173
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
338
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
310-6
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Glycol chitosan as a stabilizer for protein encapsulated into poly(lactide-co-glycolide) microparticle.
pubmed:affiliation
Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, 421 Wakara Way Suite 315, Salt Lake City, UT 84108, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't