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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2007-3-15
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pubmed:abstractText |
Expression of the Salmonella enterica serovar Typhimurium pathogenicity island 2 (SPI-2) type III secretion system is controlled by the two-component regulatory system SsrA-SsrB. We used a transcriptomic approach to help define the SsrA-SsrB regulon. We identified a gene encoding an uncharacterized effector (SseL) whose translocation into host cells depends on the SPI-2 secretion system. SseL has similarities to cysteine proteases with deubiquitinating activity. A GST-SseL fusion protein specifically hydrolyzed mono- and polyubiquitin substrates in vitro with a preference for K63-linked ubiquitin chains. Ubiquitin-modified proteins accumulated in macrophages infected with Salmonella sseL mutant strains but to a lesser extent when infected with bacteria expressing active protein, demonstrating that SseL functions as a deubiquitinase in vivo. Salmonella sseL mutant strains did not show a replication defect or induce altered levels of cytokine production upon infection of macrophages but were defective for a delayed cytotoxic effect and were attenuated for virulence in mice.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-10051653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-10094048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-10447889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-10829079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-10844662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-10880437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-10992474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-11090361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-11677609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-11736994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-11742086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-11994148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-12021365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-12218096,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-12492857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-12824352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-12864810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-12949164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-14662750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-15029200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-15120074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-15258613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-15322005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-15491354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-15571815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-15944300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-16109378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-16148945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-16177297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-16301742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-16728640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-16824101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-17158898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-2050395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-2548211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-8248143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-9119490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-9140973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-9194702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-9254655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-9294220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-9302299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-9356502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-9529323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-9573064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17360673-9786194
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
104
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3502-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17360673-Animals,
pubmed-meshheading:17360673-Mice,
pubmed-meshheading:17360673-Salmonella,
pubmed-meshheading:17360673-Endopeptidases,
pubmed-meshheading:17360673-Macrophages,
pubmed-meshheading:17360673-Membrane Proteins,
pubmed-meshheading:17360673-Immunoassay,
pubmed-meshheading:17360673-Bacterial Proteins,
pubmed-meshheading:17360673-Amino Acid Sequence,
pubmed-meshheading:17360673-Molecular Sequence Data,
pubmed-meshheading:17360673-Sequence Alignment,
pubmed-meshheading:17360673-Sequence Analysis, DNA
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