Source:http://linkedlifedata.com/resource/pubmed/id/17359977
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
2007-4-30
|
| pubmed:abstractText |
We investigated the effect of a novel ROCK inhibitor, Y-39983, on neurite regeneration in vitro and axonal regeneration in the crushed cat optic nerve in vivo. To determine the effective dose for neurite regeneration, retinal pieces were cultured with ROCK inhibitors, Y-39983 or Y-27632, a well-characterized ROCK inhibitor, and the number and length of TUJ-1-positive neurites were evaluated. The greatest number of neurites protruded at a dose of 3-10 microM Y-39983 and at a dose of 10-100 microM Y-27632, respectively. The neurite number at maximum effect of Y-39983 was greater than that of Y-27632. No significant difference was observed between values of neurite length with the inhibitors. Based on this finding, we examined the effect of Y-39983 on axonal regeneration in the crushed optic nerve in vivo. Immediately after crushing the left optic nerve, Y-39983 was injected into the vitreous and the crushed site. An injection of 10 microM Y-39983 induced the crushed axons to regenerate and pass over the crush site. In contrast, very few axons passed beyond the crush site in the optic nerve with phosphate-buffered saline injection. The second injection of 10 microM Y-39983 on day 7 doubled the number of regenerated axons, suggesting that new axons may have entered into the optic nerve after day 7 and that a continuous supply of the drug may make more axons to regenerate.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amides,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/Y 27632,
http://linkedlifedata.com/resource/pubmed/chemical/rho-Associated Kinases
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0014-4886
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
205
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
230-40
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:17359977-Amides,
pubmed-meshheading:17359977-Animals,
pubmed-meshheading:17359977-Axons,
pubmed-meshheading:17359977-Cats,
pubmed-meshheading:17359977-Cell Survival,
pubmed-meshheading:17359977-Cells, Cultured,
pubmed-meshheading:17359977-Enzyme Inhibitors,
pubmed-meshheading:17359977-Female,
pubmed-meshheading:17359977-Injections,
pubmed-meshheading:17359977-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:17359977-Male,
pubmed-meshheading:17359977-Nerve Crush,
pubmed-meshheading:17359977-Nerve Fibers,
pubmed-meshheading:17359977-Nerve Regeneration,
pubmed-meshheading:17359977-Neuroglia,
pubmed-meshheading:17359977-Optic Nerve,
pubmed-meshheading:17359977-Protein-Serine-Threonine Kinases,
pubmed-meshheading:17359977-Pyridines,
pubmed-meshheading:17359977-Retina,
pubmed-meshheading:17359977-Retinal Ganglion Cells,
pubmed-meshheading:17359977-Time Factors,
pubmed-meshheading:17359977-Vitreous Body,
pubmed-meshheading:17359977-rho-Associated Kinases
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
A novel ROCK inhibitor, Y-39983, promotes regeneration of crushed axons of retinal ganglion cells into the optic nerve of adult cats.
|
| pubmed:affiliation |
Department of Ophthalmology, Nagoya University Graduate School of Medicine, Tsuruma-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|