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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1992-3-10
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pubmed:databankReference |
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pubmed:abstractText |
The Bacillus subtilis tyrS gene, which encodes tyrosyl-tRNA synthetase (TyrTS), was isolated, and its nucleotide sequence was determined. The cloned gene was shown to complement an Escherichia coli tyrS (Ts) mutant. The predicted amino acid sequence exhibited 70% identity to that of Bacillus stearothermophilus TyrTS and 55% identity to that of E. coli TyrTS, while identity to a second cryptic B. subtilis TyrTS gene, designated tyrZ, was only 27%. Primer extension analysis indicated that tyrS transcription initiated at a vegetative promoter sequence located 300 nucleotides upstream of the AUG start codon. The mRNA leader region was found to contain an inverted repeat sequence resembling a transcriptional terminator. Expression of a transcriptional tyrS-lacZ fusion was found to be induced by starvation for tyrosine in a tyrosine auxotroph (tyrA1). Transcription initiation was unaffected by tyrosine starvation. Deletion of the terminator region in a tyrS-lacZ fusion resulted in high-level constitutive expression. Immediately preceding the putative terminator was sequence element found to be conserved in the upstream region of a number of Bacillus tRNA synthetase genes as well as in the ilv-leu biosynthetic operon; mutation of this element in tyrS resulted in low-level uninducible expression. The conservation of this sequence element suggests that aminoacyl-tRNA synthetase genes and the ilv-leu operon may be regulated by a common mechanism in Bacillus spp.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-1699930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-1806041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-1852609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-1906866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-2115870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-2126463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-2127701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-2188953,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-2203971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-2437099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-2492512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-2504923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-3011073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-3026925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-3054873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-3123885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-3149612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-3442641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-3525162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-3549464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-3960121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-5001208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-6152242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-6420647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-6761148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-6793593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-6840095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1735721-94251
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
174
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pubmed:geneSymbol |
lacZ,
tyrS,
tyrZ
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1299-306
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1735721-Amino Acid Sequence,
pubmed-meshheading:1735721-Bacillus subtilis,
pubmed-meshheading:1735721-Base Sequence,
pubmed-meshheading:1735721-Cloning, Molecular,
pubmed-meshheading:1735721-Gene Expression Regulation, Bacterial,
pubmed-meshheading:1735721-Genetic Complementation Test,
pubmed-meshheading:1735721-Molecular Sequence Data,
pubmed-meshheading:1735721-Mutation,
pubmed-meshheading:1735721-Recombinant Fusion Proteins,
pubmed-meshheading:1735721-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:1735721-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:1735721-Sequence Alignment,
pubmed-meshheading:1735721-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1735721-Temperature,
pubmed-meshheading:1735721-Tyrosine,
pubmed-meshheading:1735721-Tyrosine-tRNA Ligase,
pubmed-meshheading:1735721-beta-Galactosidase
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pubmed:year |
1992
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pubmed:articleTitle |
Analysis of the Bacillus subtilis tyrS gene: conservation of a regulatory sequence in multiple tRNA synthetase genes.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130-3932.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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