Linked Life Data

17350706

Source:http://linkedlifedata.com/resource/pubmed/id/17350706

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-4-16
pubmed:abstractText
Molecular methodologies are increasingly being developed to assess the origin and authenticity of raw organic materials and processed food products. Here we describe the application of a microarray-based assay for single nucleotide polymorphisms (SNPs) identification in olive cultivars. The assay distinguishes alleles in a ligation detection reaction (LDR), with subsequent fluorescent detection by hybridization on a universal array (UA). The LDR-UA approach was used to detect 17 SNPs in olive genomic sequences previously amplified by PCR from fresh olive leaves. Genotype calls obtained with the LDR-UA were in full agreement with those determined by direct sequencing. The panel of 17 SNPs is sufficient to discriminate 49 olive varieties selected among the most widely cultivated for olive oil production in the Mediterranean area.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0168-1656
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
129
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
565-74
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Olive variety identification by ligation detection reaction in a universal array format.
pubmed:affiliation
Institute of Biomedical Technologies, National Research Council, LITA, Via Fratelli Cervi 93, I-20090 Segrate (MI), Italy. clarissa.consolandi@itb.cnr.it
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't