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pubmed-article:1734921pubmed:abstractTextA method has been developed to allow one to extend the practical limit of oligonucleotide synthesis by coupling the synthesis reaction to a subsequent PCR. Given that DNA synthesizers are capable of producing reasonable yields of oligonucleotides that are 125-150 bases in length, this method could be used to recover the minute amount of full-length product present in mixtures extended well beyond the established limits. This technology could be applied to gene synthesis and mutagenesis.lld:pubmed
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pubmed-article:1734921pubmed:authorpubmed-author:MillerJ HJHlld:pubmed
pubmed-article:1734921pubmed:authorpubmed-author:MichaelsM LMLlld:pubmed
pubmed-article:1734921pubmed:authorpubmed-author:HsiaoH MHMlld:pubmed
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pubmed-article:1734921pubmed:volume12lld:pubmed
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pubmed-article:1734921pubmed:pagination44, 46, 48lld:pubmed
pubmed-article:1734921pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:1734921pubmed:year1992lld:pubmed
pubmed-article:1734921pubmed:articleTitleUsing PCR to extend the limit of oligonucleotide synthesis.lld:pubmed
pubmed-article:1734921pubmed:affiliationDepartment of Microbiology and Molecular Genetics, University of California, Los Angeles 90024.lld:pubmed
pubmed-article:1734921pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1734921pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed