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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1992-3-12
|
| pubmed:abstractText |
A method has been developed to allow one to extend the practical limit of oligonucleotide synthesis by coupling the synthesis reaction to a subsequent PCR. Given that DNA synthesizers are capable of producing reasonable yields of oligonucleotides that are 125-150 bases in length, this method could be used to recover the minute amount of full-length product present in mixtures extended well beyond the established limits. This technology could be applied to gene synthesis and mutagenesis.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0736-6205
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
44, 46, 48
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1734921-Base Sequence,
pubmed-meshheading:1734921-DNA,
pubmed-meshheading:1734921-DNA-Directed DNA Polymerase,
pubmed-meshheading:1734921-Molecular Sequence Data,
pubmed-meshheading:1734921-Oligonucleotides,
pubmed-meshheading:1734921-Polymerase Chain Reaction,
pubmed-meshheading:1734921-Taq Polymerase,
pubmed-meshheading:1734921-Templates, Genetic
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Using PCR to extend the limit of oligonucleotide synthesis.
|
| pubmed:affiliation |
Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|