Source:http://linkedlifedata.com/resource/pubmed/id/17347849
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2007-4-19
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| pubmed:abstractText |
The Dickkopf (Dkk) gene family of secretory modulators of canonical Wnt/beta catenin signals is involved in the control of stem cell proliferation, homeostasis and differentiation. Bioinformatic data on dkk-1/3 gene expression, indicating high expression levels in the human pancreas, led us to analyze these two proteins in adult human pancreatic tissue. Dkk-1/3 mRNA levels and protein distribution were analyzed in isolated human islets vs. the exocrine/ductal pancreatic cells and in paraffin sections of adult human pancreata. Using real time PCR only lowest amounts of dkk-1 mRNA were detectable in the endocrine fractions. Immunohistochemistry did not reveal any Dkk-1 protein in adult human pancreatic tissue. Interestingly, Dkk-3 mRNA and protein were clearly present in adult human pancreatic islets. Messenger RNA levels for Dkk-3 were significantly higher in isolated islets as compared to the exocrine/ductal fraction. Co-staining with an antibody against insulin identified the beta cells of the pancreas as the Dkk-3-positive cells. Notably, only a subset of beta cells contained Dkk-3. As shown by western blot analysis Dkk-3 seems to be proteolytically processed in beta cells. To our knowledge, this is the first study describing a molecule with which the pool of pancreatic beta cells can be further subdivided. Future studies will show whether this sub-classification of beta cells translates into functional differences.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DKK1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DKK3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0948-6143
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
127
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
513-21
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:17347849-Adult,
pubmed-meshheading:17347849-Aged,
pubmed-meshheading:17347849-Amino Acid Sequence,
pubmed-meshheading:17347849-Animals,
pubmed-meshheading:17347849-Blotting, Western,
pubmed-meshheading:17347849-COS Cells,
pubmed-meshheading:17347849-Cercopithecus aethiops,
pubmed-meshheading:17347849-Cytoplasm,
pubmed-meshheading:17347849-Female,
pubmed-meshheading:17347849-Gene Expression Profiling,
pubmed-meshheading:17347849-Humans,
pubmed-meshheading:17347849-Immunohistochemistry,
pubmed-meshheading:17347849-Insulin,
pubmed-meshheading:17347849-Insulin-Secreting Cells,
pubmed-meshheading:17347849-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:17347849-Male,
pubmed-meshheading:17347849-Middle Aged,
pubmed-meshheading:17347849-Molecular Sequence Data,
pubmed-meshheading:17347849-Pancreas,
pubmed-meshheading:17347849-Pancreas, Exocrine,
pubmed-meshheading:17347849-RNA, Messenger,
pubmed-meshheading:17347849-Sequence Homology, Amino Acid
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| pubmed:year |
2007
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| pubmed:articleTitle |
Dickkopf-3 is expressed in a subset of adult human pancreatic beta cells.
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| pubmed:affiliation |
KMT Laboratory, Innsbruck Medical University, Innrain 66, 6020, Innsbruck, Austria. Martin.Hermann@uki.at
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| pubmed:publicationType |
Journal Article
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