Linked Life Data

17346669

Source:http://linkedlifedata.com/resource/pubmed/id/17346669

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-3-20
pubmed:abstractText
Maf is a family of transcription factor proteins that is characterized by a typical bZip structure, and one of the large mafs, mafA is a strong transactivator of insulin. To explore the role of mafA in the pancreas, we modified the mafA mRNA level in vivo in mice by the RNA interference (siRNA) technique and analyzed the resulting alteration of the expressed gene profile with a microarray system. The mafA expression level in siRNA-treated mice was reduced approximately 60% compared with control-siRNA-treated animals. Microarray analysis revealed changes in the expression level of several genes in the siRNA-treated mice, with prominent down-regulated expression of the genes encoding insulin, glucagon, and adipocytokines, suggesting possible role of mafA in the pathophysiological states of impaired metabolic responses or inflammatory reactions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
356
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
129-35
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:17346669-Adiponectin, pubmed-meshheading:17346669-Animals, pubmed-meshheading:17346669-Blotting, Western, pubmed-meshheading:17346669-Complement Factor D, pubmed-meshheading:17346669-Gene Expression Profiling, pubmed-meshheading:17346669-Glucagon, pubmed-meshheading:17346669-Injections, Intravenous, pubmed-meshheading:17346669-Insulin, pubmed-meshheading:17346669-Maf Transcription Factors, Large, pubmed-meshheading:17346669-Male, pubmed-meshheading:17346669-Mice, pubmed-meshheading:17346669-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:17346669-Pancrelipase, pubmed-meshheading:17346669-RNA, Messenger, pubmed-meshheading:17346669-RNA, Small Interfering, pubmed-meshheading:17346669-RNA Interference, pubmed-meshheading:17346669-Reverse Transcriptase Polymerase Chain Reaction
pubmed:year
2007
pubmed:articleTitle
In vivo suppression of mafA mRNA with siRNA and analysis of the resulting alteration of the gene expression profile in mouse pancreas by the microarray method.
pubmed:affiliation
Institute of Geriatrics, Tokyo Women's Medical University, 2-15-1 Shibuya, Shibuya-ku, Tokyo 150-0002, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't