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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2007-3-13
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pubmed:abstractText |
Centromeres direct chromosomal inheritance by nucleating assembly of the kinetochore, a large multiprotein complex required for microtubule attachment during mitosis. Centromere identity in humans is epigenetically determined, with no DNA sequence either necessary or sufficient. A prime candidate for the epigenetic mark is assembly into centromeric chromatin of centromere protein A (CENP-A), a histone H3 variant found only at functional centromeres. A new covalent fluorescent pulse-chase labeling approach using SNAP tagging has now been developed and is used to demonstrate that CENP-A bound to a mature centromere is quantitatively and equally partitioned to sister centromeres generated during S phase, thereby remaining stably associated through multiple cell divisions. Loading of nascent CENP-A on the megabase domains of replicated centromere DNA is shown to require passage through mitosis but not microtubule attachment. Very surprisingly, assembly and stabilization of new CENP-A-containing nucleosomes is restricted exclusively to the subsequent G1 phase, demonstrating direct coupling between progression through mitosis and assembly/maturation of the next generation of centromeres.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-10639145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-10655499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-11086012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-11402065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-11514585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-11879637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-12177448,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-12196915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-12469133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-12515822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-12600307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-12672497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-15084747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-15159543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-15182667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-15226507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-15246429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-15282608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-15342555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-15475964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-15870271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-15897182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-16214339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-16412639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-16585270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-16622419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-16622420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-16925018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-17030981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-17199038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-17244537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-17339379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-17339381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-1740468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-1828250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-3558482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-5409962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-9024683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-9382804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-9382805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-9613205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17339380-9741625
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
176
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
795-805
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17339380-Autoantigens,
pubmed-meshheading:17339380-Centromere,
pubmed-meshheading:17339380-Chromatin,
pubmed-meshheading:17339380-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:17339380-DNA Replication,
pubmed-meshheading:17339380-Epigenesis, Genetic,
pubmed-meshheading:17339380-G1 Phase,
pubmed-meshheading:17339380-Humans,
pubmed-meshheading:17339380-Microtubules,
pubmed-meshheading:17339380-Mitosis,
pubmed-meshheading:17339380-Models, Genetic,
pubmed-meshheading:17339380-Recombinant Fusion Proteins
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pubmed:year |
2007
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pubmed:articleTitle |
Propagation of centromeric chromatin requires exit from mitosis.
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pubmed:affiliation |
Ludwig Institute for Cancer Research and Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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