Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-2-28
pubmed:abstractText
Culture filtrate proteins were obtained from Mycobacterium tuberculosis cultures after 7 days growth in Proskauer and Beck medium. The protein yield increased substantially to peak about the time the number of viable organisms reached its maximum level (day 8). Examination of the protein concentrate by SDS-PAGE revealed the presence of at least 12 separate protein bands varying from 10 to 90 kD. Mice were injected subcutaneously with 20 micrograms of M. tuberculosis culture filtrate (MTCF) protein suspended in saline or Freund's complete or incomplete adjuvant. The vaccinated mice were subjected to an aerogenic challenge with 10(3) colony-forming unit (CFU) M. tuberculosis Erdman and a significant reduction in the number of viable organisms was observed in the spleens and lungs determined over a 21-day period compared with age-matched normal controls. Mice immunized with the same culture filtrate proteins bound to nitrocellulose particles also showed some resistance to the virulent challenge, suggesting that individual antigens present in the culture filtrate were able to induce a protective T cell-mediated immune response in appropriately immunized mice.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-1898899, http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-1962110, http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-2507635, http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-2540520, http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-3079814, http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-3104476, http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-3128483, http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-3139828, http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-3936247, http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-4278310, http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-5002229, http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-5002230, http://linkedlifedata.com/resource/pubmed/commentcorrection/1733641-6602861
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0009-9104
pubmed:author
pubmed:issnType
Print
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
94-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Immunization of mice with mycobacterial culture filtrate proteins.
pubmed:affiliation
Trudeau Institute, Inc., Saranac Lake, NY 12983.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.