| pubmed-article:1733507 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1733507 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:1733507 | lifeskim:mentions | umls-concept:C1254426 | lld:lifeskim |
| pubmed-article:1733507 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:1733507 | lifeskim:mentions | umls-concept:C0332197 | lld:lifeskim |
| pubmed-article:1733507 | lifeskim:mentions | umls-concept:C0018284 | lld:lifeskim |
| pubmed-article:1733507 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:1733507 | lifeskim:mentions | umls-concept:C0443252 | lld:lifeskim |
| pubmed-article:1733507 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:1733507 | lifeskim:mentions | umls-concept:C0205228 | lld:lifeskim |
| pubmed-article:1733507 | lifeskim:mentions | umls-concept:C1881379 | lld:lifeskim |
| pubmed-article:1733507 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1733507 | pubmed:dateCreated | 1992-3-5 | lld:pubmed |
| pubmed-article:1733507 | pubmed:abstractText | Neither lytic NK cells nor IL-2-responsive NK precursors were produced in myeloid (Dexter) long-term bone marrow cultures (LTBMC). However, when myeloid LTBMC were switched to lymphoid (Whitlock-Witte) conditions and reseeded ("recharged") with fresh bone marrow cells (BMC), nonadherent cells with NK lytic activity and NK 1.1+ phenotype were produced within 1-2 weeks without the addition of exogenous IL-2 to the cultures. NK- and T cell-depleted BMC proliferated extensively in switched cultures and in 2 weeks generated cells that lysed the NK target YAC-1 but not the LAK target P815. The presence of NK precursors in the cultures was confirmed by reculturing nonadherent cells harvested from recharged LTBMC in fresh medium containing 50 U rIL-2/ml. High levels of NK lytic activity were generated. Sequential expression of NK 1.1 and IL-2 responsiveness followed by lytic activity was demonstrated by harvesting cells early after recharge, prior to the appearance of lytic cells. Elimination of NK 1.1+ cells depleted the ability to respond to IL-2 in secondary culture. Our studies demonstrate that myeloid-to-lymphoid switched LTBMC support the proliferation and differentiation of NK lineage cells from their NK 1.1-, nonlytic progenitors in the absence of an exogenous source of growth factors. | lld:pubmed |
| pubmed-article:1733507 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1733507 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1733507 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1733507 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1733507 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1733507 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:1733507 | pubmed:issn | 0008-8749 | lld:pubmed |
| pubmed-article:1733507 | pubmed:author | pubmed-author:RossiGG | lld:pubmed |
| pubmed-article:1733507 | pubmed:author | pubmed-author:PollackS BSB | lld:pubmed |
| pubmed-article:1733507 | pubmed:author | pubmed-author:TsujiJJ | lld:pubmed |
| pubmed-article:1733507 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1733507 | pubmed:volume | 139 | lld:pubmed |
| pubmed-article:1733507 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1733507 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1733507 | pubmed:pagination | 352-62 | lld:pubmed |
| pubmed-article:1733507 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1733507 | pubmed:meshHeading | pubmed-meshheading:1733507-... | lld:pubmed |
| pubmed-article:1733507 | pubmed:meshHeading | pubmed-meshheading:1733507-... | lld:pubmed |
| pubmed-article:1733507 | pubmed:meshHeading | pubmed-meshheading:1733507-... | lld:pubmed |
| pubmed-article:1733507 | pubmed:meshHeading | pubmed-meshheading:1733507-... | lld:pubmed |
| pubmed-article:1733507 | pubmed:meshHeading | pubmed-meshheading:1733507-... | lld:pubmed |
| pubmed-article:1733507 | pubmed:meshHeading | pubmed-meshheading:1733507-... | lld:pubmed |
| pubmed-article:1733507 | pubmed:meshHeading | pubmed-meshheading:1733507-... | lld:pubmed |
| pubmed-article:1733507 | pubmed:meshHeading | pubmed-meshheading:1733507-... | lld:pubmed |
| pubmed-article:1733507 | pubmed:meshHeading | pubmed-meshheading:1733507-... | lld:pubmed |
| pubmed-article:1733507 | pubmed:meshHeading | pubmed-meshheading:1733507-... | lld:pubmed |
| pubmed-article:1733507 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1733507 | pubmed:articleTitle | Production and differentiation of NK lineage cells in long-term bone marrow cultures in the absence of exogenous growth factors. | lld:pubmed |
| pubmed-article:1733507 | pubmed:affiliation | Department of Biological Structure, University of Washington, Seattle 98195. | lld:pubmed |
| pubmed-article:1733507 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1733507 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:1733507 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1733507 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1733507 | lld:pubmed |
