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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0017337,
umls-concept:C0024109,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0225698,
umls-concept:C0543481,
umls-concept:C1314939,
umls-concept:C1449575,
umls-concept:C1511938,
umls-concept:C1979963,
umls-concept:C2003903
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pubmed:issue |
5
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pubmed:dateCreated |
2007-3-2
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pubmed:abstractText |
Fetal breathing-like movements (FBMs) are important in normal lung growth and pneumocyte differentiation. In amyogenic mouse embryos (designated as Myf5-/-:MyoD-/-, entirely lacking skeletal musculature and FBMs), type II pneumocytes fail to differentiate into type I pneumocytes, the cells responsible for gas exchange, and the fetuses die from asphyxia at birth. Using oligonucleotide microarrays, we compared gene expression in the lungs of Myf5-/-:MyoD-/- embryos to that in normal lungs at term. Nine genes were found to be up-regulated and 54 down-regulated at least 2-fold in the lungs of double-mutant embryos. Since many down-regulated genes are involved in lymphocyte function, immunohistochemistry was employed to study T- and B-cell maturity in the thymus and spleen. Our findings of normal lymphocyte maturity implied that the down-regulation was specific to the double-mutant lung phenotype and not to its immune system. Immunostaining also revealed altered distribution of transcription and growth factors (SATB1, c-Myb, CTGF) from down-regulated genes whose knockouts are now known to undergo embryonic or neonatal death secondary to respiratory failure. Together, it appears that microarray analysis has identified a profile of genes potentially involved in pneumocyte differentiation and therefore in the mechanisms that may be implicated in the mechanochemical signal transduction pathways underlying FBMs-dependent pulmonary hypoplasia.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Connective Tissue Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Ctgf protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Attachment Region Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Myf5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/MyoD Protein,
http://linkedlifedata.com/resource/pubmed/chemical/MyoD1 myogenic differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Myogenic Regulatory Factor 5,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myb,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Satb1 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1699-5848
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
483-95
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:17330803-Animals,
pubmed-meshheading:17330803-Cell Differentiation,
pubmed-meshheading:17330803-Connective Tissue Growth Factor,
pubmed-meshheading:17330803-Gene Expression Profiling,
pubmed-meshheading:17330803-Gene Expression Regulation, Developmental,
pubmed-meshheading:17330803-Immediate-Early Proteins,
pubmed-meshheading:17330803-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:17330803-Lung,
pubmed-meshheading:17330803-Lung Diseases,
pubmed-meshheading:17330803-Lymphocytes,
pubmed-meshheading:17330803-Matrix Attachment Region Binding Proteins,
pubmed-meshheading:17330803-Mice,
pubmed-meshheading:17330803-Mice, Knockout,
pubmed-meshheading:17330803-MyoD Protein,
pubmed-meshheading:17330803-Myogenic Regulatory Factor 5,
pubmed-meshheading:17330803-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:17330803-Proto-Oncogene Proteins c-myb,
pubmed-meshheading:17330803-RNA, Messenger,
pubmed-meshheading:17330803-Reproducibility of Results,
pubmed-meshheading:17330803-Respiratory Mechanics,
pubmed-meshheading:17330803-Respiratory Muscles,
pubmed-meshheading:17330803-Time Factors
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pubmed:year |
2007
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pubmed:articleTitle |
Microarray analysis of Myf5-/-:MyoD-/- hypoplastic mouse lungs reveals a profile of genes involved in pneumocyte differentiation.
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pubmed:affiliation |
Dalhousie University, Faculty of Medicine, Department of Anatomy and Neurobiology, Halifax, NS, Canada.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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