Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2007-4-26
pubmed:abstractText
The coronavirus membrane (M) protein carboxy tail interacts with the nucleocapsid during virus assembly. Previous studies demonstrated that the two terminal residues are important, and the charged residue (R227) in the penultimate position in the mouse hepatitis coronavirus (MHV) A59 M protein was suggested to participate in intermolecular interactions with negative charges in the nucleocapsid (N) protein. To determine the significance of the positive charge at position 227, we substituted the arginine with lysine (K), aspartic acid (D), glutamic acid (E), or alanine (A) and studied these by reverse genetics in the context of a MHV full-length infectious clone. Viruses with wild-type phenotype were readily recovered with the K or A substitutions. In contrast, negative-charge substitutions were not tolerated as well. In all recovered R227D viruses the negative charge was replaced with heterologous residues resulting from apparent template switching during negative-strand synthesis of subgenomic RNA 7. An additional second-site compensatory V202I substitution was present in some viruses. Recovered R227E viruses had second-site changes within the M protein carboxy tail that were partially compensatory. Significantly, most of the second site changes in the R227E mutant viruses were previously shown to compensate for the removal of negative charges in the N protein. Our results strongly indicate that a positive charge is not absolutely required. It is clear that other regions within the tail must also be involved in helping mediate interactions between the M protein and the nucleocapsid.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-10438834, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-10756047, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-10799570, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-10933723, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-11080472, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-11152504, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-11162792, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-11533169, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-11774530, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-11967315, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-12368349, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-12584316, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-12781533, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-14694129, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-15331724, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-15681451, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-15780881, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-16227251, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-16470161, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-1660837, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-16611893, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-207820, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-2992976, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-3008826, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-3095828, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-6268741, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-6318433, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-7593163, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-7984417, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-8083990, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-8294506, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-8615041, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-8617249, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-9371586, http://linkedlifedata.com/resource/pubmed/commentcorrection/17329345-9658133
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5339-48
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Importance of the penultimate positive charge in mouse hepatitis coronavirus A59 membrane protein.
pubmed:affiliation
Biodesign Institute, Center for Infectious Diseases and Vaccinology, PO Box 875401, Arizona State University, Tempe, AZ 85287-5401, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural