| pubmed-article:17328901 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17328901 | lifeskim:mentions | umls-concept:C0006901 | lld:lifeskim |
| pubmed-article:17328901 | lifeskim:mentions | umls-concept:C1185738 | lld:lifeskim |
| pubmed-article:17328901 | lifeskim:mentions | umls-concept:C0005554 | lld:lifeskim |
| pubmed-article:17328901 | lifeskim:mentions | umls-concept:C0008550 | lld:lifeskim |
| pubmed-article:17328901 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:17328901 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:17328901 | lifeskim:mentions | umls-concept:C1958853 | lld:lifeskim |
| pubmed-article:17328901 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:17328901 | pubmed:dateCreated | 2007-3-26 | lld:pubmed |
| pubmed-article:17328901 | pubmed:abstractText | Monolithic capillary columns were prepared by thermally initiated free radical polymerisation of phenyl acrylate (PA) and 1,4-phenylene diacrylate (PDA) in the confines of 200 microm I.D. fused silica capillaries. Polymerisation was performed in the presence of 2-propanol and tetrahydrofuran (THF) as inert diluents (porogens), using alpha,alpha'-azoisobutyronitrile (AIBN) as initiator. Morphology and porosity of the resulting monoliths were comprehensively studied by scanning electron microscopy (SEM), mercury intrusion porosimetry and inverse size-exclusion chromatography (ISEC). The novel poly(phenyl acrylate-co-1,4-phenylene diacrylate) (PA/PDA) monoliths showed high mechanical stability and were successfully applied to the separation of proteins and oligodeoxynucleotides, employing reversed-phase (RP) and ion-pair reversed-phase (IP-RP) conditions, respectively. Maximum loading capacities for cytochrome c and d(pT)(16) were evaluated and found to be in the region of 200 fmol. Batch-to-batch reproducibility was determined for three independently prepared PA/PDA monolithic capillary columns. Relative standard deviations (RSDs) of retention time (t(R)) of 0.7-1.6% for proteins and 0.2-2.5% for d(pT)(12-18) proved high reproducibility of the PA/PDA supports. | lld:pubmed |
| pubmed-article:17328901 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17328901 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17328901 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17328901 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17328901 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17328901 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17328901 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17328901 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17328901 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17328901 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17328901 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:17328901 | pubmed:issn | 0021-9673 | lld:pubmed |
| pubmed-article:17328901 | pubmed:author | pubmed-author:BonnGuenther... | lld:pubmed |
| pubmed-article:17328901 | pubmed:author | pubmed-author:TrojerLukasL | lld:pubmed |
| pubmed-article:17328901 | pubmed:author | pubmed-author:LubbadSaid... | lld:pubmed |
| pubmed-article:17328901 | pubmed:author | pubmed-author:BisjakClemens... | lld:pubmed |
| pubmed-article:17328901 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17328901 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:17328901 | pubmed:volume | 1147 | lld:pubmed |
| pubmed-article:17328901 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17328901 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17328901 | pubmed:pagination | 46-52 | lld:pubmed |
| pubmed-article:17328901 | pubmed:dateRevised | 2009-1-15 | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:meshHeading | pubmed-meshheading:17328901... | lld:pubmed |
| pubmed-article:17328901 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17328901 | pubmed:articleTitle | Novel monolithic poly(phenyl acrylate-co-1,4-phenylene diacrylate) capillary columns for biopolymer chromatography. | lld:pubmed |
| pubmed-article:17328901 | pubmed:affiliation | Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 52a, 6020 Innsbruck, Austria. | lld:pubmed |
| pubmed-article:17328901 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17328901 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
