Linked Life Data

17327266

Source:http://linkedlifedata.com/resource/pubmed/id/17327266

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-4-5
pubmed:abstractText
Endogenous microRNAs (miRNAs) post-transcriptionally regulate mRNA and protein expression during tissue development and function. Whereas adaptation to environmental insults are tightly regulated in human tissues, the role of miRNAs and miRNA biogenesis proteins in this context is inadequately explored. We sought to analyse the expression of the key RNAi enzyme Argonaute2 (Ago2) and other miRNA biogenesis proteins in human trophoblasts during differentiation and in hypoxic environment. Using an in vitro analysis of primary term human trophoblasts, we identified the expression of the core miRNA biogenesis proteins in human villous trophoblasts, with expression levels unaffected by cellular differentiation. We found that the miRNA biosynthetic pathway was functional and produced miRNAs, with miR-93 up-regulated and miR-424 down-regulated in hypoxic environment. In contrast, hypoxia did not alter the expression of key miRNA machinery proteins. The pivotal miRNA processing enzyme Ago2, along with its interacting protein DP103, were expressed in normal placentas as well as in placentas from pregnancies complicated by placental hypoperfusion that resulted in fetal growth restriction. Ago2 and DP103 co-immunoprecipitated, and did not limit trophoblast response to hypoxic stress. We concluded that the core miRNA machinery proteins are expressed and functional in human trophoblasts. The influence of hypoxia on the expression of a subset of placental miRNA species is unlikely to reflect altered expression of key miRNA biogenesis proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1360-9947
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
273-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:17327266-Argonaute Proteins, pubmed-meshheading:17327266-Cell Hypoxia, pubmed-meshheading:17327266-Cells, Cultured, pubmed-meshheading:17327266-DEAD Box Protein 20, pubmed-meshheading:17327266-DEAD-box RNA Helicases, pubmed-meshheading:17327266-Eukaryotic Initiation Factor-2, pubmed-meshheading:17327266-Female, pubmed-meshheading:17327266-Gene Expression Profiling, pubmed-meshheading:17327266-Humans, pubmed-meshheading:17327266-MicroRNAs, pubmed-meshheading:17327266-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:17327266-Peptide Initiation Factors, pubmed-meshheading:17327266-RNA, Messenger, pubmed-meshheading:17327266-RNA Interference, pubmed-meshheading:17327266-RNA-Induced Silencing Complex, pubmed-meshheading:17327266-Time Factors, pubmed-meshheading:17327266-Trophoblasts
pubmed:year
2007
pubmed:articleTitle
The expression of Argonaute2 and related microRNA biogenesis proteins in normal and hypoxic trophoblasts.
pubmed:affiliation
Department of Obstetrics and Gynecology, Washington University School of Medicine, St. Louis, MO 63110, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural