Source:http://linkedlifedata.com/resource/pubmed/id/17325006
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
2007-4-20
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pubmed:abstractText |
Sec14p promotes the energy-independent transfer of either phosphatidylinositol (PtdIns) or phosphatidylcholine (PtdCho) between lipid bilayers in vitro and represents the major PtdIns/PtdCho transfer protein in the budding yeast Saccharomyces cerevisiae. Herein, we employ multi-frequency high-field electron paramagnetic resonance (EPR) to analyze the electrostatic and hydrogen-bonding microenvironments for series of doxyl-labeled PtdCho molecules bound by Sec14p in a soluble protein-PtdCho complex. A structurally similar compound, 5-doxyl stearic acid dissolved in a series of solvents, was used for experimental calibration. The experiments yielded two-component rigid limit 130- and 220-GHz EPR spectra with excellent resolution in the gx region. Those components were assigned to hydrogen-bonded and nonhydrogen-bonded nitroxide species. Partially resolved 130-GHz EPR spectra from n-doxyl-PtdCho bound to Sec14p were analyzed using this two-component model and allowed quantification of two parameters. First, the fraction of hydrogen-bonded nitroxide species for each n-doxyl-PtdCho was calculated. Second, the proticity profile along the phospholipid-binding cavity of Sec14p was characterized. The data suggest the polarity gradient inside the Sec14p cavity is a significant contributor to the driving molecular forces for extracting a phospholipid from the bilayer. Finally, the enhanced g-factor resolution of EPR at 130 and 220 GHz provides researchers with a spectroscopic tool to deconvolute two major contributions to the x-component of the nitroxide g-matrix: hydrogen-bond formation and local electrostatic effects.
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pubmed:grant | |
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17325006-10648147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17325006-10773169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17325006-12069788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17325006-12885649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17325006-14731807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17325006-15869384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17325006-16455519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17325006-4351059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17325006-4366085,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17325006-7518705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17325006-7647239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17325006-9461221
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipid Transfer Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/SEC14 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Solvents
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-3495
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
92
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3686-95
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17325006-Binding Sites,
pubmed-meshheading:17325006-Computer Simulation,
pubmed-meshheading:17325006-Electron Spin Resonance Spectroscopy,
pubmed-meshheading:17325006-Hydrogen Bonding,
pubmed-meshheading:17325006-Models, Chemical,
pubmed-meshheading:17325006-Models, Molecular,
pubmed-meshheading:17325006-Phospholipid Transfer Proteins,
pubmed-meshheading:17325006-Phospholipids,
pubmed-meshheading:17325006-Protein Binding,
pubmed-meshheading:17325006-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:17325006-Solvents,
pubmed-meshheading:17325006-Static Electricity
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pubmed:year |
2007
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pubmed:articleTitle |
Local polarity and hydrogen bonding inside the Sec14p phospholipid-binding cavity: high-field multi-frequency electron paramagnetic resonance studies.
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pubmed:affiliation |
Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695-8204, USA. Tatyana_Smirnova@ncsu.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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