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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-2-27
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pubmed:databankReference |
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pubmed:abstractText |
An antisense phosphorothioate (S)-oligonucleotide to a sequence in the intervening (I) region of the gamma 2b immunoglobulin (Ig) heavy chain gene inhibits Ig secretion by B cells stimulated with lipopolysaccharide (LPS) or LPS plus interleukin 4. It is also a striking stimulant of DNA synthesis by resting B cells. The antisense S-oligonucleotide causes a 10-20-fold increase in the expression of the gamma 2b germline transcript. Among mutants of the antisense S-oligonucleotide, some show all the effects whereas others are inactive. A similar hierarchy exists in the quantitative biological activities of mutant S-oligonucleotides and in their capacity to hybridize to the sense oligonucleotide, strongly suggesting that an I gamma 2b sequence in the RNA transcript or in the noncoding strand of the DNA is the target of the antisense S-oligonucleotide. The possible relationship of the overexpression of the germline gamma 2b transcript to the biological functions of the I gamma 2b antisense S-oligonucleotide is discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-1695667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2106137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2126196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2152964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2157139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2188677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2339132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2452184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2459714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2495537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2549631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2694945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2785916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2834063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-2836790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-3132612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-3264328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-3293213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-3499613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-3933005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-6159417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-6166712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1732418-6415170
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
175
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
597-607
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1732418-Animals,
pubmed-meshheading:1732418-B-Lymphocytes,
pubmed-meshheading:1732418-Base Sequence,
pubmed-meshheading:1732418-Blotting, Northern,
pubmed-meshheading:1732418-DNA,
pubmed-meshheading:1732418-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1732418-Female,
pubmed-meshheading:1732418-Gene Expression Regulation,
pubmed-meshheading:1732418-Genes, Immunoglobulin,
pubmed-meshheading:1732418-Immunoglobulin G,
pubmed-meshheading:1732418-Immunoglobulin Heavy Chains,
pubmed-meshheading:1732418-Lymphocyte Activation,
pubmed-meshheading:1732418-Mice,
pubmed-meshheading:1732418-Mice, Inbred BALB C,
pubmed-meshheading:1732418-Molecular Sequence Data,
pubmed-meshheading:1732418-Oligonucleotides, Antisense,
pubmed-meshheading:1732418-RNA, Messenger,
pubmed-meshheading:1732418-Thionucleotides
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pubmed:year |
1992
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pubmed:articleTitle |
An antisense oligonucleotide complementary to a sequence in I gamma 2b increases gamma 2b germline transcripts, stimulates B cell DNA synthesis, and inhibits immunoglobulin secretion.
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pubmed:affiliation |
Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article
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