Linked Life Data

17321535

Source:http://linkedlifedata.com/resource/pubmed/id/17321535

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-5-2
pubmed:abstractText
Owing to their excellent optical properties, luminescent semi-conductor quantum dots (QDs) have proven themselves to be an attractive choice in biological labeling. However, there exists the concern of cytotoxicity in using these heavy metal-based nanoparticles as molecular probes. In order to improve their general biocompatibility, CdSe/ZnS QDS are encapsulated in the natural biopolymer chitosan, forming monodisperse chitosan nanoparticles in the range of 60 nm in 1 single step. This straight forward method also allows for the synthesis of chitosan nanoparticles encapsulating multi-coloured QDs. In vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity tests on primary myoblast cells suggest that the cytotoxicity of the QDs is greatly reduced after chitosan encapsulation. At the same time, fluorescence confocal microscopy studies also prove that nanoparticles are small enough to be internalized into the myoblast cells. Our results show the ease of synthesizing biocompatible, nanometer-sized chitosan nanoparticles encapsulating QDs and their promise in biological applications such as ultra-sensitive bio-detection and labeling of biomolecules.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9797
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
310
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
464-70
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Ultrafine biocompatible chitosan nanoparticles encapsulating multi-coloured quantum dots for bioapplications.
pubmed:affiliation
Nanoscience and Nanotechnology Initiative, National University of Singapore, Singapore.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't