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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-2-18
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pubmed:abstractText |
Scatchard plot analysis of the binding of 125I-labeled heparin binding cell growth-associated factor (125I-HBGAF) to NIH 3T3 cells revealed a single class of high affinity receptors (-5000/cell) with kd of -0.6 nM. 125I-HBGAF was covalently cross-linked to the cell surface receptor on NIH 3T3 cells with disuccinimidyl suberate (DSS). Two 125I-HBGAF-cross-linked complexes of 170 kDa and 142 kDa were observed on SDS-polyacrylamide gel electrophoresis under reducing and nonreducing conditions. The 125I-HBGAF-cross-linked complex formation was completely abolished in the presence of greater than or equal to 100-fold excess of unlabeled HBGAF but not PDGF, EGF, aFGF, bFGF, or insulin. 125I-HBGAF appeared to undergo rapid internalization and relatively slow degradation following binding to the HBGAF receptor on NIH 3T3 cells. These results suggest that NIH 3T3 cells express a high affinity HBGAF receptor which shows two different estimated molecular masses of -155 kDa and -127 kDa. This high affinity HBGAF receptor was also found to express in other cell types.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
182
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
188-94
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1731778-3T3 Cells,
pubmed-meshheading:1731778-Animals,
pubmed-meshheading:1731778-Binding, Competitive,
pubmed-meshheading:1731778-Carrier Proteins,
pubmed-meshheading:1731778-Cytokines,
pubmed-meshheading:1731778-Endothelial Growth Factors,
pubmed-meshheading:1731778-Growth Substances,
pubmed-meshheading:1731778-Iodine Radioisotopes,
pubmed-meshheading:1731778-Kinetics,
pubmed-meshheading:1731778-Mice,
pubmed-meshheading:1731778-Molecular Weight,
pubmed-meshheading:1731778-Radioligand Assay,
pubmed-meshheading:1731778-Receptors, Mitogen,
pubmed-meshheading:1731778-Receptors, Vascular Endothelial Growth Factor
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pubmed:year |
1992
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pubmed:articleTitle |
Characterization of heparin-binding growth-associated factor receptor on NIH 3T3 cells.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, MO 63104.
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pubmed:publicationType |
Journal Article
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