Linked Life Data

17317253

Source:http://linkedlifedata.com/resource/pubmed/id/17317253

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-4-2
pubmed:databankReference
pubmed:abstractText
Hypoxia-inducible factor 1alpha (HIF-1alpha) is a key transcription factor that controls a variety of cellular and systemic homeostatic responses to hypoxic stress. Expression and function of HIF-1alpha have not been studied in crustaceans, which experience wide fluctuations of oxygen tensions in their aquatic environment. Here we show that an HIF-1alpha homolog, gsHIF, is present in the hypoxia-tolerant grass shrimp Palaemonetes pugio. Using RT-PCR and 3' and 5'RACE, we cloned a full-length gsHIF cDNA (3822 bp) with an open reading frame encoding a 1057 amino acid protein. Similar to vertebrate HIF-1alpha, gsHIF has one basic helix-loop-helix (bHLH) domain, two PAS domains, an oxygen-dependent degradation domain (ODD) with two proline hydroxylation motifs, and a C-terminal transactivation domain (C-TAD) with an asparagine hydroxylation motif. In addition to these conserved sequences, gsHIF has a unique 230 amino acid sequence (aa 790-1020) not found in any vertebrate HIF proteins. Phylogenetic analysis indicates that grass shrimp and vertebrate HIFs belong to distinct clades within the HIF protein family. Expression analysis shows that gsHIF is constitutively expressed under normoxic (7.5 ppm DO), moderate (2.5 ppm DO) and severe (1.5 ppm DO) hypoxic conditions. In addition to gsHIF, we cloned a fragment of a second bHLH-PAS transcription factor from the grass shrimp, which had one bHLH and two PAS domains, and an overall 68% amino acid sequence homology with Apis mellifera trachealess protein.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1096-4959
pubmed:author
pubmed:issnType
Print
pubmed:volume
147
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11-9
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Hypoxia-inducible factor, gsHIF, of the grass shrimp Palaemonetes pugio: molecular characterization and response to hypoxia.
pubmed:affiliation
Department of Coastal Sciences, University of Southern Mississippi, Ocean Springs, MS 39564, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't