Source:http://linkedlifedata.com/resource/pubmed/id/17315152
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
|
pubmed:dateCreated |
2007-2-28
|
pubmed:abstractText |
The perennial Bupleurum root is thick and woody and contains high levels of interfering compounds. Common protein extraction methods have proved refractory towards the isolation of proteins suitable for 2-DE, due to the presence of interfering compounds. A novel method for extracting proteins suitable for 2-DE was established to overcome these problems. The main characteristic of this protocol is the partitioning of the proteins into the aqueous (fraction A-2), chloroform and isoamyl alcohol phases (A-3), and the interphase (A-1). The proteins are then extracted from each of these phases. From A-1, 85% (extracted protein against total proteins) proteins could be extracted and purified. For fraction A-2, a novel phenol extraction step is employed for the extraction of proteins. Based on the well-resolved 2-DE patterns, our protein preparation is free of interfering compounds. Using these methods (A-1, A-2, and A-3-3), a total of 3662 (1526 + 1128 + 1008) spots could be separated, and a protein yield of about 1.41 mg per 1.0 g fresh root material was obtained. To our knowledge, this is the first time that a protocol for protein extraction from perennial Bupleurum root has been reported that gives reproducible results. The protocol is expected to be applicable to other recalcitrant plant tissues as well.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bupleurum root extract,
http://linkedlifedata.com/resource/pubmed/chemical/Chloroform,
http://linkedlifedata.com/resource/pubmed/chemical/Pentanols,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Water,
http://linkedlifedata.com/resource/pubmed/chemical/isopentyl alcohol
|
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0173-0835
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
28
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
871-5
|
pubmed:meshHeading |
pubmed-meshheading:17315152-Bupleurum,
pubmed-meshheading:17315152-Chloroform,
pubmed-meshheading:17315152-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:17315152-Pentanols,
pubmed-meshheading:17315152-Plant Extracts,
pubmed-meshheading:17315152-Plant Proteins,
pubmed-meshheading:17315152-Plant Roots,
pubmed-meshheading:17315152-Water
|
pubmed:year |
2007
|
pubmed:articleTitle |
A novel method of protein extraction from perennial Bupleurum root for 2-DE.
|
pubmed:affiliation |
School of Pharmacy, Fudan University, Shanghai, PR China.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|