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rdf:type |
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lifeskim:mentions |
umls-concept:C0017968,
umls-concept:C0026882,
umls-concept:C0205296,
umls-concept:C0206419,
umls-concept:C0230373,
umls-concept:C0524523,
umls-concept:C0597358,
umls-concept:C1135593,
umls-concept:C1175743,
umls-concept:C1514562,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
9
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pubmed:dateCreated |
2007-4-12
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pubmed:abstractText |
The severe acute respiratory syndrome (SARS) outbreak of 2002 and 2003 occurred as a result of zoonotic transmission. Coronavirus (CoV) found in naturally infected palm civet (civet-CoV) represents the closest genetic relative to SARS-CoV, but the degree and the determinants of cross-neutralization among these viruses remain to be investigated. Studies indicate that the receptor binding domain (RBD) of the SARS-CoV spike (S) glycoprotein contains major determinants for viral entry and neutralization. We aim to characterize the impact of natural mutations within the RBDs of civet-CoVs on viral entry and cross-neutralization. In this study, the S glycoprotein genes were recovered from naturally infected civets in central China (Hubei province), extending the geographic distribution of civet-CoV beyond the southeastern province of Guangdong. Moreover, pseudoviruses generated in our laboratory with four civet S genes, each with a distinct RBD, infected cells expressing human receptor angiotensin-converting enzyme 2, but with 90 to 95% less efficiency compared to that of SARS-CoV. These four civet S genes were also constructed as DNA vaccines to immunize mice. Immunized sera elicited against most civet S glycoproteins displayed potent neutralizing activities against autologous viruses but were much less efficient (50% inhibitory concentration, 20- to 40-fold) at neutralizing SARS-CoV and vice versa. Convalescence-phase sera from humans were similarly ineffective against the dominant civet pseudovirus. Our findings suggest that the design of SARS vaccine should consider not only preventing the reemergence of SARS-CoV but also providing cross-protection, thus interrupting zoonotic transmission of a group of genetically divergent civet CoVs of broad geographic origin.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17314167-12711465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17314167-12730500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17314167-12958366,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17314167-15780866,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17314167-16485471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17314167-16597622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17314167-16670317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17314167-17194199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17314167-8902363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17314167-9396791
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-538X
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pubmed:author |
pubmed-author:ChenZhiweiZ,
pubmed-author:DengFeiF,
pubmed-author:FangQingQ,
pubmed-author:HoDavid DDD,
pubmed-author:HuZhihongZ,
pubmed-author:JaoWW,
pubmed-author:JosKK,
pubmed-author:LiTaishengT,
pubmed-author:LinRichard DRD,
pubmed-author:WangHanzhongH,
pubmed-author:YiChristopher ECE,
pubmed-author:YuWenjieW,
pubmed-author:ZhangLinqiL
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4694-700
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17314167-Base Sequence,
pubmed-meshheading:17314167-China,
pubmed-meshheading:17314167-Cluster Analysis,
pubmed-meshheading:17314167-Cross Reactions,
pubmed-meshheading:17314167-Membrane Glycoproteins,
pubmed-meshheading:17314167-Molecular Sequence Data,
pubmed-meshheading:17314167-Mutation,
pubmed-meshheading:17314167-Neutralization Tests,
pubmed-meshheading:17314167-Phylogeny,
pubmed-meshheading:17314167-Protein Structure, Tertiary,
pubmed-meshheading:17314167-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17314167-SARS Virus,
pubmed-meshheading:17314167-Sequence Analysis, DNA,
pubmed-meshheading:17314167-Viral Envelope Proteins
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pubmed:year |
2007
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pubmed:articleTitle |
Natural mutations in the receptor binding domain of spike glycoprotein determine the reactivity of cross-neutralization between palm civet coronavirus and severe acute respiratory syndrome coronavirus.
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pubmed:affiliation |
Aaron Diamond AIDS Research Center, The Rockefeller University, New York, NY 10016, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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