Linked Life Data

1730954

Source:http://linkedlifedata.com/resource/pubmed/id/1730954

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1992-2-18
pubmed:abstractText
Patients harboring a malignant brain tumor have been described as being highly immunosuppressed, as evidenced by reduced numbers of T cells and the decreased ability of their lymphocytes to produce interleukin-2 (IL-2). In order to determine whether an intrinsic abnormality exists in the T lymphocytes of glioma patients and to evaluate what role corticosteroids may play in glioma-associated immunosuppression, in vitro T cell proliferative function in the presence of recombinant IL-2 (rIL-2) was examined in age-matched groups of normal control subjects, steroid-free patients with glial tumors, steroid-dependent patients with glial tumors, and steroid-dependent patients with nonglial cerebral tumors. The results demonstrated that, when enriched T cell populations of all brain-tumor patients were stimulated with rIL-2 and phytohemagglutinin (PHA), there were no statistically significant differences between any groups. In contrast, when T cell populations were stimulated with mitogenic combinations of phorbol ester, calcium ionophore, and rIL-2, those from steroid-dependent patients with glial tumors had a significantly lower response than those from normal control subjects, suggesting that a population of T cells capable of responding to phorbol ester/ionomycin and not PHA stimulation is inhibited by corticosteroid therapy in glioma patients. In addition, T cells of four brain-tumor patient/age-matched control subject pairs were stimulated with either phorbol ester/ionomycin or PHA for 24 hours; three of the four patients expressed low-affinity IL-2 receptor levels as high or higher than their respective control subjects, suggesting that IL-2 receptor expression in these patients may be quantitatively normal once the T cell number is corrected. Taken together, these results show that the decreased PHA responsiveness that has been previously reported in lymphocytes of glioma patients is not due to a cellular abnormality within the potentially responsive cells, but rather reflects the reduced proportion of T cells within their peripheral blood which, as a consequence, reduces the level of IL-2 production attained upon activation.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-3085
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
251-60
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:1730954-Adrenal Cortex Hormones, pubmed-meshheading:1730954-Adult, pubmed-meshheading:1730954-Aged, pubmed-meshheading:1730954-Brain Neoplasms, pubmed-meshheading:1730954-Female, pubmed-meshheading:1730954-Glioma, pubmed-meshheading:1730954-Humans, pubmed-meshheading:1730954-Immune Tolerance, pubmed-meshheading:1730954-Interleukin-2, pubmed-meshheading:1730954-Ionomycin, pubmed-meshheading:1730954-Lymphocyte Activation, pubmed-meshheading:1730954-Male, pubmed-meshheading:1730954-Middle Aged, pubmed-meshheading:1730954-Phorbol 12,13-Dibutyrate, pubmed-meshheading:1730954-Phytohemagglutinins, pubmed-meshheading:1730954-Receptors, Interleukin-2, pubmed-meshheading:1730954-Recombinant Proteins, pubmed-meshheading:1730954-T-Lymphocytes, pubmed-meshheading:1730954-Tumor Cells, Cultured
pubmed:year
1992
pubmed:articleTitle
In vitro analysis of the proliferative potential of T cells from patients with brain tumor: glioma-associated immunosuppression unrelated to intrinsic cellular defect.
pubmed:affiliation
Department of Anatomy, Virginia Commonwealth University, Medical College of Virginia, Richmond.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't