Linked Life Data

17307832

Source:http://linkedlifedata.com/resource/pubmed/id/17307832

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2007-4-20
pubmed:abstractText
To find the cause of the skinning-induced fragility of frog skeletal muscle, the transverse relaxation process of 1H-NMR signals from skinned muscle was observed. A set of four characteristic exponentials well described the process. Aside from the extremely slow exponential component (time constant T2 > 0.4 s) representing surplus solution, the process was generally slower than that in living muscle. It had larger amplitudes of slow (T2 approximately 0.15 s) and intermediate (0.03 < T2 < 0.06 s) exponentials and had smaller amplitude and faster T2 in the rapid one (T2 < 0.03 s), suggesting that skinned muscle is more sol-like than intact myoplasm. To resolve their causes, we traced the exponentials following a stepwise treatment of living whole muscle to an isolated skinned fiber. Osmotic expansion of living muscle comparable to skinned muscle increased the intermediate exponential and decreased the rapid one without affecting T2. Subsequent chemical skinning markedly increased the slow exponential, decreased the rapid one, and slowed the intermediate one. The fiber isolation had no appreciable effect. Because l-carnosine at physiological concentration could not recover the skinning-induced difference, the difference would reflect the dilution and efflux of larger macromolecules, which stabilize myoplasm as a gel.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3610-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Skinning effects on skeletal muscle myowater probed by T2 relaxation of 1H-NMR.
pubmed:affiliation
Department of Physiology, The Jikei University School of Medicine, Tokyo 105-8461, Japan. sml@jikei.ac.jp
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't