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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-2-14
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pubmed:databankReference |
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pubmed:abstractText |
Previously, we reported a rat S1 protein that is antigenically related to statin, a nonproliferating cell-specific marker; however, it shares high homology with the known human elongation factor-1 alpha (EF-1 alpha). To differentiate S1 from rat EF-1 alpha and to study their respective regulation for expression, a rat EF-1 alpha cDNA clone was isolated and characterized. The nucleotide and deduced amino acid sequences of this partial rat EF-1 alpha cDNA are compared with that of human and mouse as well as with rat S1. Both their messages were detected in rat brain by EF-1 alpha- or S1-specific probes. However, the mRNA encoding EF-1 alpha is more abundant than that encoding S1. S1 and EF-1 alpha expression were investigated in the parotid and submandibular glands of untreated rats and those treated with isoproterenol, a proliferation-inducing catecholamine. Quantitative solution hybridization demonstrated a dramatic reduction (approximately 68%) in the S1 mRNA following isoproterenol injection in proliferation-responsive parotid glands and a mild reduction (approximately 20%) of S1 steady-state messages in the proliferation-refractile submandibular glands. A slight increase or no changes of EF-1 alpha levels in both parotid and submandibular glands following isoproterenol treatment are also observed. Therefore, the EF-1 alpha and S1 genes are different genes, both expressed and regulated in vivo, but in differential quantitative and qualitative patterns.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
267
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pubmed:geneSymbol |
EF-1&agr;,
S1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
699-702
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:1730661-Amino Acid Sequence,
pubmed-meshheading:1730661-Animals,
pubmed-meshheading:1730661-Base Sequence,
pubmed-meshheading:1730661-Blotting, Northern,
pubmed-meshheading:1730661-Cell Cycle Proteins,
pubmed-meshheading:1730661-DNA,
pubmed-meshheading:1730661-Gene Expression,
pubmed-meshheading:1730661-Glyceraldehyde-3-Phosphate Dehydrogenases,
pubmed-meshheading:1730661-Isoproterenol,
pubmed-meshheading:1730661-Male,
pubmed-meshheading:1730661-Molecular Sequence Data,
pubmed-meshheading:1730661-Nuclear Proteins,
pubmed-meshheading:1730661-Nucleic Acid Hybridization,
pubmed-meshheading:1730661-Parotid Gland,
pubmed-meshheading:1730661-Peptide Elongation Factors,
pubmed-meshheading:1730661-Proteins,
pubmed-meshheading:1730661-RNA, Messenger,
pubmed-meshheading:1730661-Rats,
pubmed-meshheading:1730661-Rats, Inbred Strains,
pubmed-meshheading:1730661-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1730661-Submandibular Gland
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pubmed:year |
1992
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pubmed:articleTitle |
Characterization of the statin-like S1 and rat elongation factor 1 alpha as two distinctly expressed messages in rat.
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pubmed:affiliation |
Department of Pharmacology, University of Minnesota, Medical School, Minneapolis 55455.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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