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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1992-2-14
|
| pubmed:abstractText |
The Raf-1 proto-oncogene protein kinase can be phosphorylated and activated after stimulation of cells with insulin and a variety of other growth factors and mitogens. We recently presented evidence that insulin and certain other growth factors activated one or more Raf-1 kinase kinase activities (Lee, R.M., Rapp, U. R., and Blackshear, P.J. (1991) J. Biol. Chem. 266, 10351-10357). In the present study, four peaks of Raf-1 kinase kinase activity were identified after anion-exchange chromatography of cell lysates, and two of these were activated by insulin. Further chromatographic characterization of these two peaks of insulin-activated kinase activity indicated that they contained three apparently distinct kinase activities. Two of these activities comigrated with immunoreactive extracellular signal-regulated kinases (ERK) 1 and 2 (mitogen-activated protein kinase) through three different chromatographic separations. Both ERK1 and ERK2 phosphorylated Raf-1 with reasonably high affinity (Km for ERK1 = 90 nM; Km for ERK2 = 120 nM), and produced similar, complex phosphopeptide maps; both kinases also phosphorylated myelin basic protein. The third kinase activity also phosphorylated Raf-1 and myelin basic protein but did not comigrate exactly with either immunoreactive ERK1 or ERK2. We conclude that two and possibly three insulin-activated Raf-1 kinase kinases are members of the ERK family.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 6,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-raf
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
267
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1088-92
|
| pubmed:dateRevised |
2011-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:1730637-3T3 Cells,
pubmed-meshheading:1730637-Animals,
pubmed-meshheading:1730637-Autoradiography,
pubmed-meshheading:1730637-Blotting, Western,
pubmed-meshheading:1730637-Chromatography, Ion Exchange,
pubmed-meshheading:1730637-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1730637-Enzyme Activation,
pubmed-meshheading:1730637-Insulin,
pubmed-meshheading:1730637-Kinetics,
pubmed-meshheading:1730637-Mice,
pubmed-meshheading:1730637-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:1730637-Mitogen-Activated Protein Kinase 6,
pubmed-meshheading:1730637-Mitogen-Activated Protein Kinases,
pubmed-meshheading:1730637-Peptide Mapping,
pubmed-meshheading:1730637-Phosphorylation,
pubmed-meshheading:1730637-Protein Kinases,
pubmed-meshheading:1730637-Proto-Oncogene Proteins,
pubmed-meshheading:1730637-Proto-Oncogene Proteins c-raf
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Evidence that extracellular signal-regulated kinases are the insulin-activated Raf-1 kinase kinases.
|
| pubmed:affiliation |
Howard Hughes Medical Institute Laboratories, Duke University Medical Center, Durham, North Carolina 27710.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|