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rdf:type |
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lifeskim:mentions |
umls-concept:C0004572,
umls-concept:C0007452,
umls-concept:C0033684,
umls-concept:C0039194,
umls-concept:C0205263,
umls-concept:C0334094,
umls-concept:C0444659,
umls-concept:C0871261,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
2
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pubmed:dateCreated |
1992-2-18
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pubmed:abstractText |
A major portion of a Babesia bovis-specific gene encoding a 77-kDa merozoite protein (Bb-1) produced during natural infection in cattle and in microaerophilous culture was subcloned into the pGEX1N expression vector. Recombinant Bb-1 protein fused to glutathione S-transferase (Bb-1-GST) was used to examine cellular immune responses in B. bovis-immune cattle. Sera from rabbits immunized with Bb-1-GST reacted with fusion protein and with the native antigen present in crude B. bovis but not with B. bigemina merozoites. Bb-1-GST but not GST induced strong proliferation of T lymphocytes from these immune cattle, and Bb-1-reactive T-cell lines which consisted of a mixed population of either CD4+ and CD8+ cells or CD4+, CD8+, and "null" (gamma delta T) cells were established by in vitro stimulation of peripheral blood mononuclear cells with the recombinant fusion protein. Three CD4+ CD8- and three CD4- CD8+ Bb-1-specific T-cell clones were identified after limiting-dilution cloning of the cell lines. The studies described here demonstrate that the 77-kDa protein of B. bovis contains T-cell epitopes capable of eliciting proliferation of two types of T cells in immune cattle, an important consideration for the design of a recombinant subunit vaccine.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-15463101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-1712911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-1832875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-1902342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-1971793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2050406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2069677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2110215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2136883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2237407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2295814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2407434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2426182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2429434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2453470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2473398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2482443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2497579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2524273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2533341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2648160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2676576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2696164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-2965099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-3047011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-3061754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-3120015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-3126679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-3410541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-3930599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-3980077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-4551144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-6345394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-6391219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-6542722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-6967703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-7355284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-786161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1730498-820430
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
644-52
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1730498-Animals,
pubmed-meshheading:1730498-Babesia bovis,
pubmed-meshheading:1730498-Cattle,
pubmed-meshheading:1730498-Female,
pubmed-meshheading:1730498-Glutathione Transferase,
pubmed-meshheading:1730498-Immunization,
pubmed-meshheading:1730498-Lymphocyte Activation,
pubmed-meshheading:1730498-Protozoan Proteins,
pubmed-meshheading:1730498-Rabbits,
pubmed-meshheading:1730498-Recombinant Fusion Proteins,
pubmed-meshheading:1730498-T-Lymphocytes,
pubmed-meshheading:1730498-Vaccines, Synthetic
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pubmed:year |
1992
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pubmed:articleTitle |
Induction of proliferative responses of T cells from Babesia bovis-immune cattle with a recombinant 77-kilodalton merozoite protein (Bb-1).
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pubmed:affiliation |
Department of Veterinary Pathobiology, Texas A & M University, College Station 77843.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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