Linked Life Data

17303357

Source:http://linkedlifedata.com/resource/pubmed/id/17303357

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-4-27
pubmed:abstractText
Salvia Miltiorrhiza Bunge (SMB), a traditional Chinese medicinal herb, has been alleged to support bone healing. However, the effects of the isolated major components of SMB on osseous cells and their corresponding effective doses are still unclear. In the present study, the effects of three components of SMB, including tanshinone IIA (Ts), salvianolic acid B (salB) and protocatechuic aldehyde (Pca), on mesenchymal bone marrow cells with the potential for osteoblastic differentiation were investigated. Various concentrations of Ts, salB and Pca were added to a rat bone marrow cell culture. The total metabolic activity and differentiation of bone marrow cells were evaluated by a metabolic assay and alkaline phosphatase (ALP) activity, respectively. The morphology and number of cells was observed by phase contrast microscopy and fluorescent microscopy after propidium iodide staining, respectively. Ts suppressed the growth and differentiation of bone precursor cells. SalB exhibited a biphasic effect: the high concentration of 160 microg/mL significantly depressed the population of bone marrow cells, however, lower concentrations (3-80 microg/mL) enhanced the total metabolic activity and their ALP expression. Pca suppressed the bone marrow cell population in a dose-dependent manner. Therefore, SalB has the potential to ameliorate bone healing by stimulating both the total metabolic activity and ALP activity of osteoblastic cells. Aqueous extracts, which preferably contain salB over Pca and are free of Ts therefore are recommended for bone formation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0378-8741
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
111
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
573-83
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:17303357-Alkaline Phosphatase, pubmed-meshheading:17303357-Animals, pubmed-meshheading:17303357-Benzaldehydes, pubmed-meshheading:17303357-Benzofurans, pubmed-meshheading:17303357-Bone Marrow Cells, pubmed-meshheading:17303357-Catechols, pubmed-meshheading:17303357-Cell Differentiation, pubmed-meshheading:17303357-Cells, Cultured, pubmed-meshheading:17303357-Diterpenes, Abietane, pubmed-meshheading:17303357-Dose-Response Relationship, Drug, pubmed-meshheading:17303357-Drugs, Chinese Herbal, pubmed-meshheading:17303357-Male, pubmed-meshheading:17303357-Mesenchymal Stem Cells, pubmed-meshheading:17303357-Osteoblasts, pubmed-meshheading:17303357-Osteogenesis, pubmed-meshheading:17303357-Phenanthrenes, pubmed-meshheading:17303357-Rats, pubmed-meshheading:17303357-Rats, Sprague-Dawley, pubmed-meshheading:17303357-Salvia miltiorrhiza
pubmed:year
2007
pubmed:articleTitle
The effect of the major components of Salvia Miltiorrhiza Bunge on bone marrow cells.
pubmed:affiliation
Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't