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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2007-2-13
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pubmed:abstractText |
This study was designed to investigate the effects of polyamines on mechanical contraction and voltage-dependent calcium current (VDCC) of guinea-pig gastric smooth muscle. Mechanical contraction and calcium channel current I(Ba) were recorded by isometric tension recording and whole-cell patch clamp technique. Spermine, spermidine and putrescine inhibited spontaneous contraction of the gastric smooth muscle in a concentration-dependent manner. Spermine (2 mM) reduced high K+ (50 mM)-induced contraction to 16+/-6.4% of the control (n=9), and significantly inhibited I(Ba) in a reversible manner (p<0.05; IC50=0.8 mM). Pre- and post-treatment of tissue with spermine (2-5 mM, n=10) also inhibited acetylcholine (10 microM)-induced phasic contraction to 5+/-6.4% of the control. Inhibitory effect of spermine on I(Ba) was observed at a wide range of test potentials of current/voltage (I/V) relationship (p<0.05), and steady-state activation of I(Ba) was shifted to the right by spermine (p<0.05). Spermidine and putrescine (1 mM each) also inhibited I(Ba) to 51+/-5.7% and 81+/-5.3% of the control, respectively. And putrescine (1 mM) inhibited I(Ba) at whole tested potentials (p<0.05) without significant change of kinetics (p<0.05). Finally, 5 mM putrescine also inhibited high K+-induced contraction to 53+/-7.1% of the control (n=4). These findings suggest that polyamines inhibit contractions of guinea-pig gastric smooth muscle via inhibition of VDCC.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-10024506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-10519136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-10814501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-12193217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-1312941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-15535467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-15557285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-16428322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-2550890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-2557436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-3443558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-3708915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-3802129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-3834220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-4054214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-4726513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-6118960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-6206782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-6870914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-7177773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-7491276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-7530333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-7630314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-7810609,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-8333295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-8662289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-8843708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-8866856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-8903399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-8908205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-9950827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17297251-9972518
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1011-8934
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pubmed:author |
pubmed-author:KimDong WoonDW,
pubmed-author:KimKi WhanKW,
pubmed-author:KimSeung RyulSR,
pubmed-author:KimYoung ChulYC,
pubmed-author:KimYoung HoYH,
pubmed-author:KwonSeong-ChunSC,
pubmed-author:LeeSang JinSJ,
pubmed-author:LeeSang-JeonSJ,
pubmed-author:ParkSeon-MeeSM,
pubmed-author:SimJae HoonJH,
pubmed-author:XingDe-GangDG,
pubmed-author:XuWen-XieWX,
pubmed-author:YounSei JinSJ
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
48-56
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:17297251-Animals,
pubmed-meshheading:17297251-Calcium,
pubmed-meshheading:17297251-Calcium Channels,
pubmed-meshheading:17297251-Female,
pubmed-meshheading:17297251-Guinea Pigs,
pubmed-meshheading:17297251-Male,
pubmed-meshheading:17297251-Muscle, Smooth,
pubmed-meshheading:17297251-Muscle Contraction,
pubmed-meshheading:17297251-Polyamines,
pubmed-meshheading:17297251-Potassium,
pubmed-meshheading:17297251-Pyloric Antrum
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pubmed:year |
2007
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pubmed:articleTitle |
Effects of polyamines on contractility of guinea-pig gastric smooth muscle.
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pubmed:affiliation |
Department of Physiology, Chungbuk National University, College of Medicine, Cheongju, Korea. physiokyc@chungbuk.ac.kr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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