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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-2-12
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pubmed:abstractText |
Arachidonate 15-lipoxygenase (arachidonate:oxygen 15-oxidoreductase, EC 1.13.11.33) is a lipid-peroxidating enzyme that is implicated in oxidizing low density lipoprotein to its atherogenic form. Monocyte/macrophage 15-lipoxygenase is present in human atherosclerotic lesions. To pursue a basis for induction of the enzyme, which is not present in blood monocytes, the ability of relevant cytokines to regulate its expression was investigated. Interleukin 4 (IL-4), among 16 factors tested, specifically induced 15-lipoxygenase mRNA and protein in cultured human monocytes. Interferon gamma and hydrocortisone inhibited this induction. High-performance liquid chromatography analysis of lipid extracts from IL-4-treated monocytes detected 15-lipoxygenase products esterified to the cellular membrane lipids, indicating enzymatic action on endogenous substrates. Stimulation of IL-4-treated monocytes with calcium ionophore or opsonized zymosan A enhanced the formation of 15-lipoxygenase products. These data identify IL-4 and interferon gamma as physiological regulators of lipoxygenase expression and suggest an important link between 15-lipoxygenase function and the immune/inflammatory response in atherosclerosis as well as other diseases.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-1690536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-1696810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-1698286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-1846310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-1850411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2010530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2010531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2018830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2116236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2116308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2117277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2120232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2162888,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2166915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2248430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2302735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2318885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2343305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2505620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2536929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2648148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2820055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-2877743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-3027181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-3098292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-3138949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-3139813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-3202857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-3258008,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-3486902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729692-6435125
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/15-hydroxy-5,8,11,13-eicosatetraenoi...,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 15-Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxyeicosatetraenoic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
217-21
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1729692-Arachidonate 15-Lipoxygenase,
pubmed-meshheading:1729692-Arachidonic Acid,
pubmed-meshheading:1729692-Blotting, Northern,
pubmed-meshheading:1729692-Cytokines,
pubmed-meshheading:1729692-Enzyme Induction,
pubmed-meshheading:1729692-Fluorescent Antibody Technique,
pubmed-meshheading:1729692-Gene Expression,
pubmed-meshheading:1729692-Humans,
pubmed-meshheading:1729692-Hydroxyeicosatetraenoic Acids,
pubmed-meshheading:1729692-Inflammation,
pubmed-meshheading:1729692-Interferon-gamma,
pubmed-meshheading:1729692-Interleukin-4,
pubmed-meshheading:1729692-Membrane Lipids,
pubmed-meshheading:1729692-Monocytes,
pubmed-meshheading:1729692-RNA, Messenger
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pubmed:year |
1992
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pubmed:articleTitle |
Specific inflammatory cytokines regulate the expression of human monocyte 15-lipoxygenase.
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pubmed:affiliation |
Cardiovascular Research Institute, University of California, San Francisco 94143-0911.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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