Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-2-12
pubmed:abstractText
A successful test is reported to generate long-range contiguous coverage of DNA from a human cytogenetic band in overlapping yeast artificial chromosomes (YACs). Seed YACs in band Xq26 were recovered from a targeted library of clones from Xq24-q28 with 14 probes, including probes for the hypoxanthine guanine phosphoribosyltransferase- and coagulation factor IX-encoding genes and nine probes used in linkage mapping. Neighboring YACs were then identified by 25 "walking" steps with end-clones, and the content of 71 probes in cognate YACs was verified by further hybridization analyses. The resultant contig extends across 8 million base pairs, including most of band Xq26, with an order of markers consistent with linkage data. YAC-based mapping, thus, permits steps toward a fully integrated physical and genetic map and is probably adequate to sustain most of the human genome project.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-1990831, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2004771, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2006154, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2037299, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2073838, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2081601, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2122561, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2173146, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2218515, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2294758, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2341149, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2365355, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2510296, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2537978, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2544027, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2678105, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2690083, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2771952, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2846283, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-2937300, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-3033825, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-3045566, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-3294162, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-3538420, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-3556729, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-4000947, http://linkedlifedata.com/resource/pubmed/commentcorrection/1729687-6247908
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
89
pubmed:geneSymbol
F9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
177-81
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Yeast artificial chromosomes spanning 8 megabases and 10-15 centimorgans of human cytogenetic band Xq26.
pubmed:affiliation
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't