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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1992-2-13
|
| pubmed:abstractText |
Immunoaffinity chromatography with a monoclonal antibody produced against bovine tau protein was used to purify tau proteins from human brain. Fifty grams of brain tissue yielded approximately 2 mg of pure tau proteins. The affinity-purified human tau was used to produce a high-titered rabbit anti-human tau serum. The monoclonal anti-tau antibody and the polyclonal rabbit anti-tau serum were then used to construct a sandwich enzyme-linked immunosorbent assay for detection of human tau proteins, with a sensitivity of 1 ng/ml.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-3042
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
58
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
548-53
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:1729400-Animals,
pubmed-meshheading:1729400-Antibodies, Monoclonal,
pubmed-meshheading:1729400-Chromatography, Affinity,
pubmed-meshheading:1729400-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1729400-Humans,
pubmed-meshheading:1729400-Immune Sera,
pubmed-meshheading:1729400-tau Proteins
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Affinity purification of human tau proteins and the construction of a sensitive sandwich enzyme-linked immunosorbent assay for human tau detection.
|
| pubmed:affiliation |
Innogenetics, Ghent, Belgium.
|
| pubmed:publicationType |
Journal Article
|