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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1992-2-13
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pubmed:abstractText |
Transcriptionally active regions of the Haloferax volcanii genome were mapped by hybridization of radiolabeled cDNA to Southern blots of our minimal set of overlapping cosmid clones covering 96% of the 4.1-Mbp genome. Transcription during exponential growth occurred in nearly every region of the 2,920-kbp chromosome. Large parts of the 690- and the 86-kbp plasmids were transcribed, but the 440-kbp plasmid showed little expression. Transcription after a 40-min heat shock at 65 degrees C was generally reduced, apart from a small set of strongly expressed loci all situated on the chromosome.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
174
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30-4
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:1729217-Archaea,
pubmed-meshheading:1729217-Bacterial Proteins,
pubmed-meshheading:1729217-Blotting, Southern,
pubmed-meshheading:1729217-Cell Division,
pubmed-meshheading:1729217-Chromosome Mapping,
pubmed-meshheading:1729217-Cloning, Molecular,
pubmed-meshheading:1729217-Cosmids,
pubmed-meshheading:1729217-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:1729217-Genome, Bacterial,
pubmed-meshheading:1729217-Heat-Shock Proteins,
pubmed-meshheading:1729217-Transcription, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
Transcriptionally active regions in the genome of the archaebacterium Haloferax volcanii.
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pubmed:affiliation |
Department of Biology, University of Ottawa, Ontario, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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