Linked Life Data

17292138

Source:http://linkedlifedata.com/resource/pubmed/id/17292138

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-2-12
pubmed:abstractText
Carbon nanotubes have widespread applications, although little is known about their toxicity or interaction with cells. Recent studies in skin and lung reveal that carbon nanoparticles can cause toxicity. To generate a preliminary protein profile of nanotube exposure, we analyzed human epidermal keratinocytes (HEKs) exposed to multi-walled carbon nanotubes (MWCNTs) in cell culture using large-format, two-dimensional (2D) gel electrophoresis and mass spectrometry (MS). Compared with controls, 24 hours of MWCNT exposure altered the expression of 36 proteins (P < .01), whereas 106 were altered at 48 hours. At both time points, roughly 67% of the affected proteins were significantly down-regulated. Peptide mass fingerprinting identified most of the differentially expressed proteins, and the various protein identities reflected a complex cellular response to MWCNT exposure. In addition to proteins associated with metabolism, cell signaling, and stress, we observed a consistent effect on the expression of cytoskeletal elements and vesicular trafficking components. These data clearly show that MWCNTs are capable of altering protein expression in a target epithelial cell that constitutes a primary route of occupational exposure for manufactured nanotubes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1549-9642
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
158-68
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Multi-walled carbon nanotube exposure alters protein expression in human keratinocytes.
pubmed:affiliation
Department of Cellular and Integrative Physiology, Biotechnology Research and Training Center, Indiana University School of Medicine, Indianapolis, Indiana, USA. fwitzman@iupui.edu <fwitzman@iupui.edu>
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't