Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-2-9
pubmed:abstractText
In the mammalian small intestine, coupled NaCl absorption occurs via the dual operation of Na/H and Cl/HCO(3) exchange on the villus cell brush border membrane (BBM). Although constitutive nitric oxide (cNO) has been demonstrated to alter gastrointestinal tract functions, how cNO may specifically alter these two transporters to regulate coupled NaCl absorption is unknown. In villus cells, inhibition of cNO synthase (cNOS) with l-N(G)-nitroarginine methylester (l-NAME) stimulated Na/H exchange whereas Cl/HCO(3) exchange was unaffected. In villus cell BBM vesicles (BBMV) prepared from rabbits treated with l-NAME, Na/H exchange was also stimulated. d-NAME, an inactive analog of l-NAME, and N(6)-(1-imonoethyl)-l-lysine dihydrochloride, a more selective inhibitor of inducible NO synthase, did not affect Na/H exchange. Kinetic studies demonstrated that the mechanism of stimulation is secondary to an increase in the maximal rate of uptake of Na, without an alteration in the affinity of the transporter for Na. Northern blot studies demonstrated an increase in the message for the BBM Na/H exchanger NHE3, and Western blot studies showed that the immunoreactive protein levels of NHE3 was increased when cNOS was inhibited. Thus these results indicate that cNO under nominal physiological states most likely maintains an inhibitory tone on small intestinal coupled NaCl absorption by specifically inhibiting BBM Na/H expression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Ammonium Chloride, http://linkedlifedata.com/resource/pubmed/chemical/Chloride-Bicarbonate Antiporters, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Lysine, http://linkedlifedata.com/resource/pubmed/chemical/N(6)-(1-iminoethyl)lysine, http://linkedlifedata.com/resource/pubmed/chemical/NG-Nitroarginine Methyl Ester, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II, http://linkedlifedata.com/resource/pubmed/chemical/Sodium, http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter, http://linkedlifedata.com/resource/pubmed/chemical/sodium-hydrogen exchanger 3
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0193-1857
pubmed:author
pubmed:issnType
Print
pubmed:volume
292
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
G475-81
pubmed:dateRevised
2007-12-3
pubmed:meshHeading
pubmed-meshheading:17290013-Ammonium Chloride, pubmed-meshheading:17290013-Animals, pubmed-meshheading:17290013-Cell Membrane, pubmed-meshheading:17290013-Chloride-Bicarbonate Antiporters, pubmed-meshheading:17290013-Enteritis, pubmed-meshheading:17290013-Enzyme Inhibitors, pubmed-meshheading:17290013-Epithelial Cells, pubmed-meshheading:17290013-Gene Expression, pubmed-meshheading:17290013-Hydrogen-Ion Concentration, pubmed-meshheading:17290013-Intestinal Mucosa, pubmed-meshheading:17290013-Kinetics, pubmed-meshheading:17290013-Lysine, pubmed-meshheading:17290013-Microvilli, pubmed-meshheading:17290013-NG-Nitroarginine Methyl Ester, pubmed-meshheading:17290013-Nitric Oxide, pubmed-meshheading:17290013-Nitric Oxide Synthase, pubmed-meshheading:17290013-Nitric Oxide Synthase Type II, pubmed-meshheading:17290013-Rabbits, pubmed-meshheading:17290013-Sodium, pubmed-meshheading:17290013-Sodium-Hydrogen Antiporter
pubmed:year
2007
pubmed:articleTitle
Mechanism of regulation of rabbit intestinal villus cell brush border membrane Na/H exchange by nitric oxide.
pubmed:affiliation
Section of Digestive Diseases, West Virginia Univ School of Medicine, Morgantown, WV 26506-9161, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural