Source:http://linkedlifedata.com/resource/pubmed/id/17289848
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2007-4-20
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| pubmed:abstractText |
Follicular development and ovulation are suppressed during lactation in various mammalian species, mainly due to the suppression of pulsatile GnRH/LH secretion. Metastin (kisspeptin-54), a KiSS-1 gene product, is an endogenous ligand for GPR54, a G-protein-coupled receptor, and suggested to play a critical role in regulating the gonadal axis. The present study therefore aims to determine whether metastin (kisspeptin-54)-GPR54 signaling in discrete brain areas is inhibited by the suckling stimulus that causes suppression of LH secretion in lactating rats. Quantitative RT-PCR revealed that the KiSS-1 mRNA level was significantly lower in the arcuate nucleus (ARC)-median eminence region in lactating ovariectomized (OVX) and estrogen-treated OVX rats than in nonlactating controls. KiSS-1 mRNA in the anteroventral periventricular nucleus was kept at a low level in both lactating and nonlactating rats despite estrogen treatment. GPR54 mRNA levels were significantly lower in lactating than nonlactating rats in the anteroventral periventricular nucleus, but the levels in lactating mothers of the preoptic area and ARC-median eminence were comparable with nonlactating controls. Although KiSS-1 mRNA-expressing cells or metastin (kisspeptin-54) immunoreactivities were densely located in the ARC of nonlactating controls, few were found in the ARC of lactating OVX animals. Various doses of metastin (kisspeptin-54) (0.02, 0.2, and 2 nmol) injected into the third ventricle caused a significant increase in LH secretion in both lactating and nonlactating OVX rats, suggesting that lactating rats are responsive to metastin (kisspeptin-54) stimulus. Thus, the present study demonstrated that KiSS-1 mRNA/metastin (kisspeptin-54) expression is inhibited in the ARC by the suckling stimulus, suggesting that the inhibition is most probably involved in suppressing LH secretion in lactating rats.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Kiss1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Kiss1r protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Kisspeptins,
http://linkedlifedata.com/resource/pubmed/chemical/Luteinizing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0013-7227
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
148
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2226-32
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:17289848-Animals,
pubmed-meshheading:17289848-Animals, Suckling,
pubmed-meshheading:17289848-Arcuate Nucleus,
pubmed-meshheading:17289848-Female,
pubmed-meshheading:17289848-Immunohistochemistry,
pubmed-meshheading:17289848-In Situ Hybridization,
pubmed-meshheading:17289848-Injections, Intraventricular,
pubmed-meshheading:17289848-Kisspeptins,
pubmed-meshheading:17289848-Lactation,
pubmed-meshheading:17289848-Luteinizing Hormone,
pubmed-meshheading:17289848-Median Eminence,
pubmed-meshheading:17289848-Neurons,
pubmed-meshheading:17289848-Ovulation Inhibition,
pubmed-meshheading:17289848-Proteins,
pubmed-meshheading:17289848-RNA, Messenger,
pubmed-meshheading:17289848-Rats,
pubmed-meshheading:17289848-Rats, Wistar,
pubmed-meshheading:17289848-Receptors, G-Protein-Coupled,
pubmed-meshheading:17289848-Signal Transduction,
pubmed-meshheading:17289848-Third Ventricle
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| pubmed:year |
2007
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| pubmed:articleTitle |
Inhibition of metastin (kisspeptin-54)-GPR54 signaling in the arcuate nucleus-median eminence region during lactation in rats.
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| pubmed:affiliation |
Laboratory of Reproductive Science, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
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| pubmed:publicationType |
Journal Article
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